Recombinant Human B7-H6 His-tag Protein, CF

R&D Systems | Catalog # 9309-B7

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human B7-H6 His-tag Protein (9309-B7)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q68D85

Applications

Bioactivity
View all B7-H6 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human B7-H6 protein
Asp25-Ser262, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp25

Predicted Molecular Mass

28 kDa

SDS-PAGE

51-63 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-H6 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) that produces 50% of the optimal binding response is 0.02-0.1 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9309-B7 in the following applications:

Scientific Data Images for Recombinant Human B7-H6 His-tag Protein, CF

Recombinant Human B7-H6 His-tag Protein Bioactivity

Recombinant Human B7-H6 His-tag Protein Bioactivity

When Recombinant Human B7-H6 (Catalog # 9309-B7) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) binds with an ED50 of 0.02-0.1 µg/mL.

Formulation, Preparation, and Storage

9309-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: B7-H6

B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins (1  2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).

References

  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
  4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
  5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
  6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
  7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.

Long Name

B7 Homolog 6

Alternate Names

B7H6, DKFZp686I21167, DKFZp686O24166, NCR3LG1

Entrez Gene IDs

374383 (Human); 102121659 (Cynomolgus Monkey)

Gene Symbol

NCR3LG1

UniProt

Q68D85

Additional B7-H6 Products

Product Documents for Recombinant Human B7-H6 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human B7-H6 His-tag Protein, CF

For research use only

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