>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6
is immobilized at 1 µg/mL
(100 µL/well), the concentration of
Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK)
that produces 50% of the optimal binding response is 0.02-0.1 μg/mL.
Human embryonic kidney cell, HEK293-derived Asp25-Ser262, with a C-terminal 6-His tag
When Recombinant Human B7-H6 (Catalog # 9309-B7) isimmobilized at 1 μg/mL (100 μL/well), Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) binds with an ED50 of 0.02-0.1 μg/mL.
is a glycosylated member of the B7 family of immune co-stimulatory
proteins (1 2). Mature human B7-H6 consists of a 238 amino acid (aa)
extracellular domain (ECD) that contains one Ig-like V domain and one
Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa
cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif
(3). Both of the Ig-like domains carry N-linked glycosylation (4).
Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence
identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and
53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and
rat have not been identified. The Ig-like V domain mediates 1:1
stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5).
It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of
NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3).
B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and
melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on
tumor cells correlates with tumor cell sensitivity to NKp30‑dependent
cell lysis (7).
Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
Li, Y. et al. (2011) J. Exp. Med. 208:703.
Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
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