Recombinant Human B7-H6 His-tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human NKp30/NCR3 Fc Chimera (Catalog # 1849-NK) that produces 50% of the optimal binding response is 0.02-0.1 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Asp25-Ser262, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Asp25
  • Predicted Molecular Mass
    28 kDa
  • SDS-PAGE
    51-63 kDa, reducing conditions
9309-B7
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human B7-H6 (Catalog # 9309-B7) isimmobilized at 1 μg/mL (100 μL/well), Recombinant Human NKp30/NCR3 Fc Chimera (Catalog #
1849-NK) binds with an ED50 of 0.02-0.1 μg/mL.
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co-stimulatory proteins (1  2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53%-56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
  • References:
    1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
    2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
    3. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
    4. Li, Y. et al. (2011) J. Exp. Med. 208:703.
    5. Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
    6. Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
    7. Byrd, A. et al. (2007) PLoS ONE 2:e1339.
  • Long Name:
    B7 Homolog 6
  • Entrez Gene IDs:
    374383 (Human); 102121659 (Cynomolgus Monkey)
  • Alternate Names:
    B7 homolog 6; B7H6; B7-H6; DKFZp686I21167; DKFZp686O24166; NCR3LG1
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Biotinylated Antibody

WB BAM050 3  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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