Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

Newer Version Available: 2406-BR
NEW
A New rh BMPR-IA is Available! ~2 fold better activity; Lower endotoxin specification; Enhanced purity!

Carrier Free

Catalog # Availability Size / Price Qty
315-BR-100/CF

With Carrier

Catalog # Availability Size / Price Qty
315-BR-100
R&D Systems Recombinant Proteins and Enzymes
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Citations (11)
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Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit rhBMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1‑0.3 µg/mL in the presence of 30 ng/mL of recombinant human BMP-4.
Source
Mouse myeloma cell line, NS0-derived human BMPR-IA/ALK-3 protein
Human BMPR-IA
(Gln24 - Arg152)
Accession # CAA80257
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln24 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
41.5 kDa (monomer)
SDS-PAGE
55 kDa, reducing conditions

Product Datasheets

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315-BR (with carrier)

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315-BR/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

315-BR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

315-BR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: BMPR-IA/ALK-3

Bone Morphogenetic Protein Receptor IA (BMPR-IA), also known as ALK-3, BRK-1, and CD292, is a glycosylated 60 - 65 kDa type I receptor in the TGF‑ beta serine/threonine kinase receptor family (1 ‑ 3). Binding of TGF-beta superfamily ligands induces formation of a heterotetrameric complex that contains two chains each of a type I and a type II receptor in multiple combinations. The type II receptors phosphorylate the type I receptors which then phosphorylate and activate Smad signal transduction proteins (1, 2). Mature human BMPR-IA consisits of a 129 amino acid (aa) extracellular domain (ECD), a 24 aa transmembrane segment, and a 356 aa cytoplasmic region that contains the tyrosine kinase domain (4). Within the ECD, human BMPR-IA shares 98% and 97% aa sequence identity with mouse and rat BMPR-IA, respectively. BMPR-IA is involved in the development and function of a wide range of tissues. During early embryogenesis it is required for migration of the anterior visceral endoderm (AVE) and proper development of the anterior-posterior axis (5). Tissue-specific conditional knockout experiments have demonstrated the importance of BMPR-IA in the development and morphogenesis of the heart, lung, palate, teeth, and mandible (6 - 8). In the adult, BMPR-IA plays a role in glucose-stimulated insulin secretion by pancreatic beta cells, osteoclast activity and bone remodeling, reactive astrocyte-mediated scar formation following spinal cord injury, and ovulation and fertility (9 - 12).

References
  1. Wu, M.Y. and C.S. Hill (2009) Dev. Cell 16:329.
  2. Nickel, J. et al. (2009) Cytokine Growth Factor Rev. 20:367.
  3. de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
  4. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  5. Miura, S. et al. (2010) Dev. Biol. 341:246.
  6. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.
  7. Sun, J. et al. (2008) Am. J. Pathol. 172:571.
  8. Li, L. et al. (2011) Dev. Biol. 349:451.
  9. Goulley, J. et al. (2007) Cell Metab. 5:207.
  10. Kamiya, N. et al. (2008) J. Bone Miner. Res. 23:2007.
  11. Sahni, V. et al. (2010) J. Neurosci. 30:1839.
  12. Edson, M.A. et al. (2010) Mol. Endocrinol. 24:1251.
Long Name
Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs
657 (Human); 12166 (Mouse)
Alternate Names
ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; BMPRIA; BMPR-IA; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3

Citations for Recombinant Human BMPR-IA/ALK-3 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. BMPR1A is necessary for chondrogenesis and osteogenesis while BMPR1B prevents hypertrophic differentiation
    Authors: T Mang, K Kleinschmi, F Ploeger, A Schoeneman, S Lindemann, A Gigout
    J. Cell. Sci., 2020;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  2. Serum induces transcription of Hey1 and Hey2 genes by Alk1 but not Notch signaling in endothelial cells.
    Authors: Woltje K, Jabs M, Fischer A
    PLoS ONE, 2015;10(3):e0120547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis.
    Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A
    PLoS ONE, 2015;10(6):e0131453.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  5. Inhibition of bone morphogenetic protein signaling attenuates anemia associated with inflammation.
    Authors: Steinbicker AU, Sachidanandan C, Vonner AJ
    Blood, 2011;117(18):4915-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
    Authors: Sengle G, Ono RN, Lyons KM, Bachinger HP, Sakai LY
    J. Mol. Biol., 2008;381(4):1025-39.
  7. BMP4 regulation of human megakaryocytic differentiation is involved in thrombopoietin signaling.
    Authors: Jeanpierre S, Nicolini FE, Kaniewski B, Dumontet C, Rimokh R, Puisieux A, Maguer-Satta V
    Blood, 2008;112(8):3154-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.
    Authors: David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
    Blood, 2007;109(5):1953-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Regulation of ADMP and BMP2/4/7 at opposite embryonic poles generates a self-regulating morphogenetic field.
    Authors: Reversade B, De Robertis EM
    Cell, 2005;123(6):1147-60.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Receptor Binding Assay
  10. Specification of dorsal telencephalic character by sequential Wnt and FGF signaling.
    Authors: Gunhaga L, Marklund M, Sjodal M, Hsieh JC, Jessell TM, Edlund T
    Nat. Neurosci., 2003;6(7):701-7.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. Langerin-expressing dendritic cells in human tissues are related to CD1c+ dendritic cells and distinct from Langerhans cells and CD141high XCR1+ dendritic cells.
    Authors: Bigley V, McGovern N, Milne P, Dickinson R, Pagan S, Cookson S, Haniffa M, Collin M
    J Leukoc Biol, 0;97(4):627-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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