Recombinant Human BTN3A2 Protein, CF

R&D Systems | Catalog # 9514-BT

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human BTN3A2 Protein (9514-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P78410-1

Applications

Bioactivity
View all BTN3A2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human BTN3A2 protein
Gln30-Trp248, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln30

Predicted Molecular Mass

24 kDa

SDS-PAGE

26-35 kDa, reducing conditions

Activity

Measured by its ability to enhance anti-CD3-induced IFN-gamma secretion of mouse CD3+ T cells.
The ED50 for this effect is 0.5-2.5 μg/mL.

Scientific Data Images for Recombinant Human BTN3A2 Protein, CF

Recombinant Human BTN3A2 Protein Bioactivity

Recombinant Human BTN3A2 Protein Bioactivity

Recombinant Human BTN3A2 (Catalog # 9514-BT) enhances anti-CD3 antibody induced IFN-gamma secretion by mouse CD3+T cells. The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation, and Storage

9514-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BTN3A2

BTN3A2 (Butyrophilin subfamily 3 member A2; also BTF3 and BT3.2) is a 36 kDa (predicted) glycoprotein, member of the BTN family, Ig Superfamily of molecules. It is postulated to be expressed on immune-related cells, as it has a structural similarity to MHC and CD80/CD86 molecules. Mature human BTN3A2 is a 305 amino acid (aa) type I transmembrane protein. It contains a 219 aa extracellular region with one V-type Ig-like domain and a 65 aa cytoplasmic tail. The cytoplasmic region undergoes phosphorylation on two serines. There are three potential splice forms. A rodent counterpart to BTN3A2 has not been reported. BTN3A2 mRNA over‑expression was associated with a good prognosis in relation to disease-free and overall survival in a cohort of 55 epithelial ovarian cancer (EOC) patients (1). Another study in a larger cohort of 199 high-grade EOC patients further confirmed that the protein expression of BTN3A2 in ovarian cancer tissues is positively correlated with the intraepithelial infiltration of CD4+ and CD8+ T cells (2), suggesting that BTN3A2 was a co-stimulatory molecule to modulate the infiltration of immune cells and thus the anti-cancer immunity. In consistent with previous publications, our in-house studies on BTN3A2 showed that BTN3A2 co-stimulated anti‑CD3 induced IFN-gamma secretion on CD3+ cells.

References

  1. LePage C, et al. Cancer Epidemiol Biomarkers Prev (2008) 17:913.
  2. LePage C, et al. PLoSOne (2012) 7:e38541.

Long Name

Butyrophilin, Subfamily 3, Member A2

Alternate Names

BT3.2, BT3.3, BTF4

Entrez Gene IDs

11118 (Human); 294268 (Rat)

Gene Symbol

BTN3A2

UniProt

P78410-1

Additional BTN3A2 Products

Product Documents for Recombinant Human BTN3A2 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human BTN3A2 Protein, CF

For research use only

Customer Reviews for Recombinant Human BTN3A2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human BTN3A2 Protein, CF and earn rewards!

Have you used Recombinant Human BTN3A2 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes