Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF

Catalog # Availability Size / Price Qty
10014-BT-050
Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF Bioactivity
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Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1.5-7.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human BTNL10 protein
Human BTNL10
(Ser27-Gly149)
Accession # A8MVZ5
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
40.6 kDa
SDS-PAGE
48-55 kDa & 96-108 kDa (non-reducible dimer), reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10014-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human BTNL10 Fc Chimera (Catalog # 10014-BT) inhibitsIFN-gamma secretion by human peripheral blood mononuclear cells in the presence ofanti-CD3 antibody. The ED50 for this effect is 1.5-7.5 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human BTNL10 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 48-55 kDa and 95-110 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTNL10

Butyrophilin-like 10 (BTNL10) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL10 is a 291 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region, and a short cytoplasmic domain. The ECD of human BTNL10 features a single IgV and a poorly defined IgC domain. The IgV domain of human BTNL10 shares 53% and 52% sequence identity with the equivalent ECD domain of mouse and rat BTNL10, respectively. Although just recently identified, BTNL10 is one of only five butyrophilins conserved between human and mouse (2). While the complete immunological function of BTN/BTNL molecules is only beginning to emerge, they have been shown to be important in immunity by regulating T cell function (2-4). Recent efforts have focused on BTN/BTNL as potential therapeutic targets for a wide range of diseases (2-4). Currently, both the expression pattern and native function of BTNL10 remain unknown. Our data indicate that BTNL10 inhibits the human T cell activation, including IL-2, IFN- gamma secretion, and T cell proliferation.

References
  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2012) Immunogenetics. 64:781.
  3. Abeler-Dörner L. et al. (2012) Trends Immunol. 33(1):34.
  4. Arnett, H.A. and Viney J.L. (2014) Nat Rev Immunol. 14(8):559.
Long Name
Butyrophilin-like 10
Entrez Gene IDs
100129094 (Human); 192194 (Mouse)
Alternate Names
BTN4; BTNL10 butyrophilin-like 10; BTNL10; BUTR1

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