Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF

R&D Systems | Catalog # 10014-BT

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein (10014-BT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

A8MVZ5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
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Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human BTNL10 protein
Human BTNL10
(Ser27-Gly149)
Accession # A8MVZ5		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

40.6 kDa

SDS-PAGE

48-55 kDa & 96-108 kDa (non-reducible dimer), reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1.5-7.5 μg/mL.

Scientific Data Images for Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF

Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein Bioactivity

Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein Bioactivity

Recombinant Human BTNL10 Fc Chimera (Catalog # 10014-BT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 1.5-7.5 μg/mL.
Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein SDS-PAGE

Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein SDS-PAGE

2 μg/lane of Recombinant Human BTNL10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-55 kDa and 95-110 kDa, respectively.

Formulation, Preparation, and Storage

10014-BT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

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Background: BTNL10

Butyrophilin-like 10 (BTNL10) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL10 is a 291 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region, and a short cytoplasmic domain. The ECD of human BTNL10 features a single IgV and a poorly defined IgC domain. The IgV domain of human BTNL10 shares 53% and 52% sequence identity with the equivalent ECD domain of mouse and rat BTNL10, respectively. Although just recently identified, BTNL10 is one of only five butyrophilins conserved between human and mouse (2). While the complete immunological function of BTN/BTNL molecules is only beginning to emerge, they have been shown to be important in immunity by regulating T cell function (2-4). Recent efforts have focused on BTN/BTNL as potential therapeutic targets for a wide range of diseases (2-4). Currently, both the expression pattern and native function of BTNL10 remain unknown. Our data indicate that BTNL10 inhibits the human T cell activation, including IL-2, IFN- gamma secretion, and T cell proliferation.

References

  1. Arnett, H.A. et al. (2007) J. Immunol. 178:1523.
  2. Arnett, H.A. et al. (2012) Immunogenetics. 64:781.
  3. Abeler-Dörner L. et al. (2012) Trends Immunol. 33(1):34.
  4. Arnett, H.A. and Viney J.L. (2014) Nat Rev Immunol. 14(8):559.

Long Name

Butyrophilin-like 10

Alternate Names

BTN4, BUTR1

Entrez Gene IDs

100129094 (Human); 192194 (Mouse)

Gene Symbol

BTNL10

UniProt

A8MVZ5

Additional BTNL10 Products

Product Documents for Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF

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Product Specific Notices for Recombinant Human BTNL10/Butyrophilin-like 10 Fc Protein, CF

For research use only

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