Recombinant Human BTNL3 Fc Chimera Protein, CF

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Catalog # Availability Size / Price Qty
9658-BT-050
Recombinant Human BTNL3 Fc Chimera Protein, CF Bioactivity
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Recombinant Human BTNL3 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BTNL3 protein
Human BTNL3
(Gln18-Ser237)
Accession # Q6UXE8-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19
Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass
51 kDa
SDS-PAGE
53-63 kDa, reducing conditions

Product Datasheets

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Bioactivity Recombinant Human BTNL3 Fc Chimera Protein, CF Bioactivity View Larger

Recombinant Human BTNL3 Fc Chimera (Catalog # 9658‑BT) inhibits IFN‑gamma secretion by human peripheral blood mononuclear cells in thepresence of anti-CD3 antibody. The ED50 for this effect is 1‑6 μg/mL.

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Background: BTNL3

Butyrophilin-like 3 (BTNL3) is a member of the BTN/MOG Ig-superfamily which is coded by the BTNL3 gene, also known as COLF4100 (1). BTNL3 is a 466 amino acid (aa) type I transmembrane protein that contains a 220 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 208 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in BTN/BTNL proteins, but the IgC domain is relatively weak (2-5). The ECD of human BTNL3 shares only 37% and 34% aa sequence identity with mouse (referred to as BTNL1) and rat, respectively. Human BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (7). The specific function of BTNL3, however, has yet to be fully elucidated. R&D in house data indicate that BTNL3 protein inhibits the secretion of IFN-gamma from anti-CD3 activated human T cells.

References
  1. Shibui, A. et al. (1999) J. Hum. Genet. 44:249.
  2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  3. Arnett, H.A. et al. (2009) Cytokine 46:370.
  4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  5. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  6. Guo, Y and Wang, AY (2015) Front. Immunol. 6:421.
  7. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.
Long Name
Butyrophilin-like 3
Entrez Gene IDs
10917 (Human); 100038862 (Mouse); 407781 (Rat)
Alternate Names
BTN9.1; BTNL3; BTNLR; BTNLRButyrophilin-like receptor; butyrophilin-like 3; butyrophilin-like protein 3; COLF4100

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