Recombinant Human BTNL3 Fc Chimera Protein, CF

    
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  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human BTNL3 protein
    Human BTNL3
    (Gln18-Ser237)
    Accession # Q6UXE8-1
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln18 inferred from enzymatic pyroglutamate treatment revealing Trp19
  • Structure / Form

    Disulfide-linked homodimer

  • Predicted Molecular Mass
    51 kDa
  • SDS-PAGE
    53-63 kDa, reducing conditions
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9658-BT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Human BTNL3 Fc Chimera (Catalog # 9658‑BT) inhibits IFN‑gamma secretion by human peripheral blood mononuclear cells in thepresence of anti-CD3 antibody. The ED50 for this effect is 1‑6 μg/mL.
Background: BTNL3
Butyrophilin-like 3 (BTNL3) is a member of the BTN/MOG Ig-superfamily which is coded by the BTNL3 gene, also known as COLF4100 (1). BTNL3 is a 466 amino acid (aa) type I transmembrane protein that contains a 220 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 208 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in BTN/BTNL proteins, but the IgC domain is relatively weak (2-5). The ECD of human BTNL3 shares only 37% and 34% aa sequence identity with mouse (referred to as BTNL1) and rat, respectively. Human BTNL3 mRNA has been identified in many tissues including small intestine, colon, liver, lung, bone marrow and spleen tissue, and it is highly expressed in neutrophils (2-6). BTNL3 expression has also been found to be down-regulated in colon cancer tumors (7). The specific function of BTNL3, however, has yet to be fully elucidated. R&D in house data indicate that BTNL3 protein inhibits the secretion of IFN-gamma from anti-CD3 activated human T cells.
  • References:
    1. Shibui, A. et al. (1999) J. Hum. Genet. 44:249.
    2. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
    3. Arnett, H.A. et al. (2009) Cytokine 46:370.
    4. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
    5. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
    6. Guo, Y and Wang, AY (2015) Front. Immunol. 6:421.
    7. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.
  • Long Name:
    Butyrophilin-like 3
  • Entrez Gene IDs:
    10917 (Human); 100038862 (Mouse); 407781 (Rat)
  • Alternate Names:
    BTN9.1; BTNL3; BTNLR; BTNLRButyrophilin-like receptor; butyrophilin-like 3; butyrophilin-like protein 3; COLF4100
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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