Recombinant Human BTNL9 Fc Chimera Protein, CF

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9659-BT-050
Recombinant Human BTNL9 Fc Chimera Protein Bioactivity
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Recombinant Human BTNL9 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 fot this effect is 3-15 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human BTNL9 protein
Human BTNL9
(Ser35-Lys256)
Accession # Q6UXG8-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser35
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52 kDa
SDS-PAGE
60-69 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9659-BT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human BTNL9 Fc Chimera Protein Bioactivity View Larger

Recombinant Human BTNL9 Fc Chimera (Catalog # 9659-BT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 3-15 μg/mL.

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Background: BTNL9

Butyrophilin-like 9 (BTNL9) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL9 is a 535 amino acid (aa) type I transmembrane glycoprotein that contains a 222 aa extracellular domain (ECD), a 21 aa helical transmembrane domain, and a 258 aa cytoplasmic domain. The ECD features both an IgV and IgC domain, which is commonplace in the BTN/BTNL proteins, but the IgC domain is relatively weak (1-4). The ECD of human BTNL9 shares 66% and 67% aa sequence identity with rat and mouse, respectively. Human BTNL9 mRNA has been identified in adipose, lung, thymus, spleen, colon, and cardiac tissues, but its highest levels of expression were found in B cells (2, 5). Although the specific function of BTNL9 has yet to be fully elucidated, recombinant BTNL9-Fc has been shown to bind to many immune cells including T cells, B cell, macrophages, and dendritic cells (6). BTNL9 expression has also been found to be down-regulated in colon cancer tumors (5). R&D in house data indicate that BTNL9 protein inhibits the secretion of IFN-gamma from anti-CD3 activated PMBC.

References
  1. Arnett, H.A. et al. (2014) Nat Rev Immunol. 14:5596.
  2. Arnett, H.A. et al. (2009) Cytokine 46:370.
  3. Abeler-Dorner, L. et al. (2012) Trends Immunol. 33:34.
  4. Rhodes, D. et al. (2016) Annu. Rev. Immunol. 34:151.
  5. Lebrero-Fernandez, C. et al. (2016) Immun. Inflammation Dis. 4:191.
  6. Yamazaki, T. et al. (2010) J. Immunol. 185:5907.
Long Name
Butyrophilin-like 9
Entrez Gene IDs
153579 (Human); 237754 (Mouse); 686977 (Rat)
Alternate Names
BTN3; BTNL9; butyrophilin 3; butyrophilin-like 9; butyrophilin-like protein 9; UNQ1900/PRO4346; VDLS1900

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