Recombinant Human CA125/MUC16 Protein, CF

Catalog # Availability Size / Price Qty
5609-MU-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (10)
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Recombinant Human CA125/MUC16 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin‑3 (Catalog # 1154-GA) that produces 50% of the optimal binding response is found to be approximately 0.35-1.4 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CA125/MUC16 protein
Met13360-Gln14347 (Met13472Thr & Gln13957Lys) with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met13360
Predicted Molecular Mass
110 kDa
SDS-PAGE
170-200 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5609-MU

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CA125/MUC16

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy (1, 2). Expression of isoforms, proteolytic cleavage, and heavy N- and O- linked glycosylation produce forms of human MUC16 that can vary from 1148 to 22152 amino acids (aa) in length and 200 - 5000 kDa in size (1, 2). The 22152 aa form contains ser/thr-rich N-terminal tandem repeats, 4 LRR (Leu-rich repeat) domains, 56 SEA (sea urchin sperm protein, enterokinase and agrin) domains, a transmembrane domain, and a 31 aa cytoplasmic domain that includes a tyrosine phosphorylation site (1-4). SEA domains are ~120 aa in length, contain conserved residues including potential O-glycosylation sites and a pair of cysteines, and are often found in transmembrane mucins (3). The protein produced by R&D Systems represents aa 13360-14347 of the full sequence and includes the last 6 SEA domains. It shares 68% aa identity with canine MUC16. MUC16 is over-expressed by tumor cells including ovarian and mesothelial cancers (5). The transmembrane form can adhere to mesothelin in the peritoneum, facilitating metastasis of ovarian cancer to the peritoneal cavity (5-7). MUC16 also binds galectin-1 on immune cells and enhances its expression on tumor cells (8). MUC16-expressing tumors adhere to NK cells, down-regulate CD16 and suppress NK response, which may promote immune evasion (9, 10). MUC16 is also cyclically expressed in the endometrium and may contribute to immune privilege during pregnancy (10). In the eye, MUC16 and other mucins protect the cornea and keep it hydrated. It is altered on the conjunctival epithelium of patients with non-Sjogren dry eye syndrome (11).

References
  1. Yin, B. W. T. K. O. Lloyd, 2001, J. Biol. Chem. 276:27371.
  2. Maeda, T. et al. (2004) J. Biol. Chem. 279:13174.
  3. Fendrick, J. L. et al. (1997) Tumour Biol. 18:278.
  4. Swissprot accession Q8WXI7.
  5. Kaneko, O. et al. (2009) J. Biol. Chem. 284:3739.
  6. Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
  7. Gubbels, J. A. A. et al. (2006) Mol. Cancer 5:50.
  8. Seelenmeyer, C. et al. (2003) J. Cell Sci. 116:1305.
  9. Patankar, M. S. et al. (2005) Gynecol. Oncol. 99:704.
  10. Belisle, J. A. et al. (2007) Immunology 122:418.
  11. Blalock, T. D. et al. (2007) Invest. Ophthalmol. Vis. Sci. 48:4509.
Long Name
Ovarian Cancer Antigen CA125
Entrez Gene IDs
94025 (Human)
Alternate Names
CA125 ovarian cancer antigen; CA125; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125

Citations for Recombinant Human CA125/MUC16 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Structure-guided mutagenesis of a mucin-selective metalloprotease from Akkermansia muciniphila alters substrate preferences
    Authors: DJ Shon, D Fernandez, NM Riley, MJ Ferracane, CR Bertozzi
    The Journal of Biological Chemistry, 2022;0(0):101917.
    Species: Akkermansia muciniphila
    Sample Types: Protein
    Applications: Bioassay
  2. Label-Free Detection and Spectrometrically Quantitative Analysis of the Cancer Biomarker CA125 Based on Lyotropic Chromonic Liquid Crystal
    Authors: H Shaban, MJ Lee, W Lee
    Biosensors, 2021;11(8):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. A Single-Substrate Biosensor with Spin-Coated Liquid Crystal Film for Simple, Sensitive and Label-Free Protein Detection
    Authors: PC Wu, CP Pai, MJ Lee, W Lee
    Biosensors, 2021;11(10):.
    Species: N/A
    Sample Types: Film
    Applications: ELISA
  4. Validation of Serum Biomarkers That Complement CA19-9 in Detecting Early Pancreatic Cancer Using Electrochemiluminescent-Based Multiplex Immunoassays
    Authors: J Song, LJ Sokoll, DW Chan, Z Zhang
    Biomedicines, 2021;9(12):.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  5. Highly Sensitive Detection of CA 125 Protein with the Use of an n-Type Nanowire Biosensor
    Authors: KA Malsagova, TO Pleshakova, RA Galiullin, AF Kozlov, ID Shumov, VP Popov, FV Tikhonenko, AV Glukhov, VS Ziborov, OF Petrov, VE Fortov, AI Archakov, YD Ivanov
    Biosensors, 2020;10(12):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  6. The mucin-selective protease StcE enables molecular and functional analysis of human cancer-associated mucins
    Authors: SA Malaker, K Pedram, MJ Ferracane, BA Bensing, V Krishnan, C Pett, J Yu, EC Woods, JR Kramer, U Westerlind, O Dorigo, CR Bertozzi
    Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):.
    Species: Bacteria - E. Coli
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery
    Authors: JK Fierle, J Abram-Sali, M Brioschi, M deTiani, G Coukos, SM Dunn
    Sci Rep, 2019;9(1):12815.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. Establishment of patient-derived tumor xenograft models of epithelial ovarian cancer for pre-clinical evaluation of novel therapeutics
    Clin Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA (Standard)
  9. Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.
    Authors: Fischer N, Elson G, Magistrelli G, Dheilly E, Fouque N, Laurendon A, Gueneau F, Ravn U, Depoisier J, Moine V, Raimondi S, Malinge P, Di Grazia L, Rousseau F, Poitevin Y, Calloud S, Cayatte P, Alcoz M, Pontini G, Fagete S, Broyer L, Corbier M, Schrag D, Didelot G, Bosson N, Costes N, Cons L, Buatois V, Johnson Z, Ferlin W, Masternak K, Kosco-Vilbois M
    Nat Commun, 2015;6(0):6113.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
    Authors: Prassas, Ioannis, Brinc, Davor, Farkona, Sofia, Leung, Felix, Dimitromanolakis, Apostolo, Chrystoja, Caitlin, Brand, Randall, Kulasingam, Vathany, Blasutig, Ivan M, Diamandis, Elefther
    Clin Chem, 2014;60(2):381-8.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot

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