Recombinant Human Carboxylesterase 1/CES1 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (6)

Recombinant Human Carboxylesterase 1/CES1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >6,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human Carboxylesterase 1/CES1 protein
His19-Glu563, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
61 kDa
61 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 50 mM Tris, pH 7.5
  • Recombinant Human Carboxylesterase 1/CES1 (rhCES1) (Catalog # 4920-CE)
  • Substrate: 4-Nitrophenyl acetate (4-NPA) (Sigma, Catalog # N-8130), 100 mM stock in Acetone
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhCES1 to 0.8 ng/µL in Assay Buffer.
  2. Dilute Substrate to 2 mM in deionized water.
  3. In a plate load 50 μL of rhCES1 to wells and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL Substrate and 50 µL Assay Buffer.
  4. Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)

     *Adjusted for Substrate Blank

     **Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).

Per Well:
  • rhCES1: 0.040 µg
  • Substrate: 1 mM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Carboxylesterase 1/CES1

Carboxylesterase 1 is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES-1 shares the serine hydrolase fold observed in other esterases (3). CES-1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus. The purified rhCES-1 lacks this signal, resulting in its secretion.

  1. Redinbo, M. R. and Potter, P.M. (2005) Drug Discovery Today 10:313.
  2. Satoh, T. and Hosokawa, M. (2006) Chem. Biol. Interactions 162:195.
  3. Fleming, C. D. et al. (2007) Biochemistry 46:5603.
Entrez Gene IDs
1066 (Human); 12623 (Mouse); 29225 (Rat)
Alternate Names
ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; carboxylesterase 2 (liver); CEH; CES1; Ces-1; CES1A2; CES2EC; cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1; Egasyn; HMSE1; HMSECES1A1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; MGC117365; Monocyte/macrophage serine esterase; PCE-1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; Ses-1; SES1TGH; TGH; Triacylglycerol hydrolase

Citations for Recombinant Human Carboxylesterase 1/CES1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Functions of human olfactory mucus and age-dependent changes
    Authors: T Shirai, D Takase, J Yokoyama, K Nakanishi, C Uehara, N Saito, A Kato-Namba, K Yoshikawa
    Scientific Reports, 2023;13(1):971.
    Species: Human
    Sample Types: Saliva
    Applications: Bioassay
  2. Systematic identification of biomarker-driven drug combinations to overcome resistance
    Authors: MG Rees, L Brenan, M do Carmo, P Duggan, B Bajrami, M Arciprete, A Boghossian, E Vaimberg, SJ Ferrara, TA Lewis, D Rosenberg, T Sangpo, JA Roth, VK Kaushik, F Piccioni, JG Doench, DE Root, CM Johannesse
    Nature Chemical Biology, 2022;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  3. Discovery and molecular basis of subtype-selective cyclophilin inhibitors
    Authors: AA Peterson, AM Rangwala, MK Thakur, PS Ward, C Hung, IR Outhwaite, AI Chan, DL Usanov, VK Mootha, MA Seeliger, DR Liu
    Nature Chemical Biology, 2022;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Activation of Tenofovir Alafenamide and Sofosbuvir in the Human Lung and Its Implications in the Development of Nucleoside/Nucleotide Prodrugs for Treating SARS-CoV-2 Pulmonary Infection
    Authors: J Li, S Liu, J Shi, HJ Zhu
    Pharmaceutics, 2021;13(10):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Enzyme Activity
  5. Hydrolytic Fate of 3/15-Acetyldeoxynivalenol in Humans: Specific Deacetylation by the Small Intestine and Liver Revealed Using in Vitro and ex Vivo Approaches
    Authors: el H Ajandouz, S Berdah, V Moutardier, T Bege, DJ Birnbaum, J Perrier, E Di Pasqual, M Maresca
    Toxins (Basel), 2016;8(8):.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Bioassay
  6. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977.
    Authors: Murakami E, Tolstykh T, Bao H, Niu C, Steuer HM, Bao D, Chang W, Espiritu C, Bansal S, Lam AM, Otto MJ, Sofia MJ, Furman PA
    J. Biol. Chem., 2010;285(45):34337-47.
    Species: Synthetic
    Sample Types: Small Molecule
    Applications: Enzyme Assay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human Carboxylesterase 1/CES1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Carboxylesterase 1/CES1 Protein, CF and earn rewards!

Have you used Recombinant Human Carboxylesterase 1/CES1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review