Recombinant Human CCL1/I-309 Protein

R&D Systems | Catalog # 272-I

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CCL1/I-309 Protein (272-I)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P22362.1

Applications

Bioactivity
View all CCL1/I-309/TCA-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CCL1/I-309/TCA-3 protein
Lys24-Lys96

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys24

Predicted Molecular Mass

8.5 kDa

Activity

Measured by its ability to chemoattract BW5147 mouse T lymphoma cells.
The ED50 for this effect is 3-9 ng/mL.

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8.
The ED50 for this effect is 1.5-7.5 ng/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 272-I in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 272-I
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 272-I/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma / delta T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References

  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Gene Symbol

CCL1

UniProt

P22362.1

Additional CCL1/I-309/TCA-3 Products

Product Documents for Recombinant Human CCL1/I-309 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 272-I (with carrier)
Download SDS 272-I/CF (carrier free)

Product Specific Notices for Recombinant Human CCL1/I-309 Protein

For research use only

Citations for Recombinant Human CCL1/I-309 Protein

Customer Reviews for Recombinant Human CCL1/I-309 Protein (2)

4.5 out of 5
2 Customer Ratings
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  • Recombinant Human CCL1/I-309 Protein
    Name: Anonymous
    Application: Characterization prior to downstream assays.
    Verified Customer | Posted 11/04/2019
    Recombinant Human CCL1/I-309 Protein 272-I
  • Recombinant Human CCL1/I-309 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/22/2017
    MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.
    Recombinant Human CCL1/I-309 Protein 272-I

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