Recombinant Human CCL1/I-309 Protein

  (8 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (8)
FAQs
Reviews
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to chemoattract BW5147 mouse T lymphoma cells. The ED50 for this effect is 3-9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR8. The ED50 for this effect is 1.5-7.5 ng/mL.
  • Source
    E. coli-derived human CCL1/I-309/TCA-3 protein
    Lys24-Lys96
  • Accession #
  • N-terminal Sequence
    Analysis
    Lys24
  • Predicted Molecular Mass
    8.5 kDa
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
272-I
 
272-I/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma /δ T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes.

CCL1 cDNA encodes a 96 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid residue mature protein. The CCL1 sequence contains one potential N-linked glycosylation site and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15 - 16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

  • References:
    1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci USA 89:2950.
    2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.
  • Entrez Gene IDs:
    6346 (Human); 20290 (Mouse)
  • Alternate Names:
    C-C motif chemokine 1; CCL1; chemokine (C-C motif) ligand 1; I-309; inflammatory cytokine I-309; P500; SCYA1; SCYA1Small-inducible cytokine A1; SCYA2; SISe; small inducible cytokine A1 (I-309, homologous to mouse Tca-3); T lymphocyte-secreted protein I-309; TCA3; TCA-3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. CCR8(+)FOXp3(+) Treg cells as master drivers of immune regulation
    Authors: Y Barsheshet, G Wildbaum, E Levy, A Vitenshtei, C Akinseye, J Griggs, SA Lira, N Karin
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells.
    Authors: Islam SA, Chang DS, Colvin RA
    Nat. Immunol., 2011;12(2):167-77.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. CC chemokine ligand 1 is released into the airways of atopic asthmatics.
    Authors: Montes-Vizuet R, Vega-Miranda A, Valencia-Maqueda E, Negrete-Garcia MC, Velasquez JR, Teran LM
    Eur. Respir. J., 2006;28(1):59-67.
    Species: Human
    Sample Type: BALF
    Application: Bioassay
  5. Genetic fusions with viral chemokines target delivery of nonimmunogenic antigen to trigger antitumor immunity independent of chemotaxis.
    Authors: Ruffini PA, Biragyn A, Coscia M, Harvey LK, Cha SC, Bogen B, Kwak LW
    J. Leukoc. Biol., 2004;76(1):77-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
    Authors: Cashman J, Clark-Lewis I, Eaves A, Eaves C
    Blood, 2002;99(3):792-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
    Authors: Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
    J. Leukoc. Biol., 2001;70(4):617-23.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard

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Average Rating: 4 (Based on 1 review)

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Immunoassay Standard Recombinant Human CCL1/I-309 Protein 272-I Array 272-I
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 Immunoassay Standard Anonymous 11/22/2017
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Immunoassay Standard Recombinant Human CCL1/I-309 Protein 272-I
Immunoassay Standard: Recombinant Human CCL1/I-309 Protein [272-I].

Summary

Application Immunoassay Standard

Other Experimental Details

Other Experimental Details MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.

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