Recombinant Human CCL15/MIP-1 delta/LKN-1 (68 aa) Protein

Carrier Free

Catalog # Availability Size / Price Qty
628-LK-025/CF

With Carrier

Catalog # Availability Size / Price Qty
628-LK-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (4)
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Recombinant Human CCL15/MIP-1 delta/LKN-1 (68 aa) Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 2-4 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED50 for this effect is 0.6-3 ng/mL.
Source
E. coli-derived human CCL15/MIP-1 delta protein
Ser46-Ile113
Accession #
N-terminal Sequence
Analysis
Ser46
Predicted Molecular Mass
7.4 kDa

Product Datasheets

628-LK (with carrier)

628-LK/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

628-LK

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

628-LK/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL15/MIP-1 delta

CCL15, also named Leukotactin-1 (LKN-1), MIP-5, HCC-2, and NCC-3, is a novel human CC chemokine whose gene was mapped to human chromosome 17 adjacent to the HCC-1 gene. CCL15/LKN-1, together with mouse C10, mouse MIP-1 gamma  and human MPIF-1, constitute a subgroup of CC chemokines which contain six instead of four conserved cysteine residues. The two additional cysteine residues in CCL15/LKN-1 have been shown to form a third disulfide bond CCL15/LKN-1 cDNA encodes a 113 amino acid (aa) residue precursor protein with a putative signal peptide of 21 aa residues that is cleaved to generate a 92 aa residue mature protein. In recombinant CCL15/LKN-1 preparations produced in insect cells and in yeast, amino-terminal truncations were found to have occurred. The major forms of CCL15/LKN-1 secreted by insect cells and yeast were reported to be proteins of 68 and 66 aa residues, respectively. The full length and the amino-terminal truncated forms of human CCL15δ/LKN-1 have been shown to be potent chemoattractants for monocytes and T-lymphocytes. These proteins can also chemoattract eosinophils and have been shown to induce calcium flux in human CCR1 transfected cells. Additionally, CCL15/LKN-1 can suppress colony formation by human granulocyte-macrophage, erythroid, and multipotential progenitor cells stimulated by combinations of growth factors. As a chemoattractant for THP-1 cells, R&D Systems’ 68 aa residue truncated form of CCL15 is approximately 50-fold more active than the 92 aa residue full length CCL15/LKN-1.

References
  1. Youn, B.-S. et al. (1997) J. Immunol. 159:5201.
  2. Pardigol, A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6308.
  3. Wang, W. et al. (1998) J. Clinical Immunol.18:214.
  4. Coulin, F. et al. (1997) Eur. J. Biochem. 248:507.
Entrez Gene IDs
6359 (Human)
Alternate Names
CCL15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; Leukotactin 1; Leukotactin-1; LKN1; LKN-1; Macrophage inflammatory protein 5; MIP1 delta; MIP-1 delta; MIP5; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; Small-inducible cytokine A15

Citations for Recombinant Human CCL15/MIP-1 delta/LKN-1 (68 aa) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1.
    Authors: Jang SW, Kim YS, Kim YR, Sung HJ, Ko J
    J. Biol. Chem., 2007;282(15):11092-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential chemokine activation of CC chemokine receptor 1-regulated pathways: ligand selective activation of Galpha 14-coupled pathways.
    Authors: Tian Y, New DC, Yung LY, Allen RA, Slocombe PM, Twomey BM, Lee MM, Wong YH
    Eur. J. Immunol., 2004;34(3):785-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 0;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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