Recombinant Human CCL17/TARC Protein

(15 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to chemoattract CEM‑NKr human natural killer cell resistant T-lymphoblastoid cells. Howell, D.N. et al. (1985) J. Immunol. 134:971. The ED50 for this effect is 3-9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR4. The ED50 for this effect is 2‑6 ng/mL.
  • Source
    E. coli-derived Ala24-Ser94
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala24
  • Predicted Molecular Mass
    8 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
364-DN
 
364-DN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC

CCL17 is a novel CC chemokine identified using a signal sequence trap method. CCL17 cDNA encodes a highly basic 94 amino acid (aa)residue precursor protein with a 23 aa residue signal peptide that is cleaved to generate the 71 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24 - 29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3 and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. CCL17 is constitutively expressed in thymus, and at a lower level in lung, colon, and small intestine. CCL17 is also transiently expressed in stimulated peripheral blood mononuclear cells. Recombinant CCL17 has been shown to be chemotactic for T cell lines but not monocytes or neutrophils. CCL17 was identified to be a specific functional ligand for CCR4, a receptor that is selectively expressed on T cells.

  • References:
    1. Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
    2. Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
    3. Nomiyama, H. et al. (1997) Genomics 40:211.
  • Entrez Gene IDs:
    6361 (Human); 20295 (Mouse)
  • Alternate Names:
    ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Gain-of-function CCR4 mutations in adult T cell leukemia/lymphoma.
    Authors: Nakagawa, Masao, Schmitz, Roland, Xiao, Wenming, Goldman, Carolyn, Xu, Weihong, Yang, Yandan, Yu, Xin, Waldmann, Thomas A, Staudt, Louis M
    J Exp Med, 2014;211(13):2497-505.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Lung transplantation affects expression of the chemokine receptor type 4 on specific T cell subsets.
    Authors: Paantjens AW, van de Graaf EA, Kwakkel-van Erp JM, Hoefnagel T, van Kessel DA, van den Bosch JM, Otten HG
    Clin. Exp. Immunol., 2011;166(1):103-9.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  4. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  6. CCR4-expressing T cell tumors can be specifically controlled via delivery of toxins to chemokine receptors.
    Authors: Baatar D, Olkhanud P, Newton D, Sumitomo K, Biragyn A
    J. Immunol., 2007;179(3):1996-2004.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: viral
    Sample Type: HSV-1 virion
    Application: Binding Assay
  8. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Der p, IL-4, and TGF-beta cooperatively induce EGFR-dependent TARC expression in airway epithelium.
    Authors: Heijink IH, Marcel Kies P, van Oosterhout AJ, Postma DS, Kauffman HF, Vellenga E
    Am. J. Respir. Cell Mol. Biol., 2006;36(3):351-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.
    Authors: Liu L, Jarjour NN, Busse WW, Kelly EA
    Am. J. Respir. Crit. Care Med., 2004;169(10):1118-24.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  12. TARC augments TNF-alpha-induced CTACK production in keratinocytes.
    Authors: Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
    Exp. Dermatol., 2004;13(9):551-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.
    Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping
    Nat Med, 2004;10(9):942-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
    Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
    J. Neurochem., 2002;81(2):257-69.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Binding Study
  15. Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.
    Authors: Thompson SD, Luyrink LK, Graham TB, Tsoras M, Ryan M, Passo MH, Glass DN
    J. Immunol., 2001;166(11):6899-906.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
Expand to show all 15 Citations

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