Recombinant Human CCL19/MIP-3 beta Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
361-MI-025/CF
Product Details
Citations (29)
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Recombinant Human CCL19/MIP-3 beta Protein, CF Summary

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 0.1-0.3 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR7. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived human CCL19/MIP-3 beta protein
Gly22-Ser98
Accession #
N-terminal Sequence
Analysis
Gly22
Predicted Molecular Mass
8.8 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

361-MI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

361-MI

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL19/MIP-3 beta

MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta  has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. Recombinant MIP-3 beta has been shown to be chemotactic for cultured human lymphocytes. MIP-3 beta has also been shown to be a unique functional ligand for CCR-7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

References
  1. Yoshida, R. et al. (1997) J. Biol. Chem. 272:13803.
Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

Citations for Recombinant Human CCL19/MIP-3 beta Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. A CD4+ T cell population expanded in lupus blood provides B cell help through interleukin-10 and succinate
    Authors: S Caielli, DT Veiga, P Balasubram, S Athale, B Domic, E Murat, R Banchereau, Z Xu, M Chandra, CH Chung, L Walters, J Baisch, T Wright, M Punaro, L Nassi, K Stewart, J Fuller, D Ucar, H Ueno, J Zhou, J Banchereau, V Pascual
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation
    Authors: D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka
    Sci Rep, 2017;7(1):8536.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis
    Authors: RA Raposo, M de Mulder, D Paquin-Pro, PM Brailey, VD Cabido, PM Zdinak, AS Thomas, SH Huang, GA Beckerle, RB Jones, DF Nixon
    JCI Insight, 2017;2(1):e85811.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Expression of COBLL1 encoding novel ROR1 binding partner is robust predictor of survival in chronic lymphocytic leukemia
    Authors: H Plešingero, P Janovská, A Mishra, L Smy?ková, L Poppová, A Libra, K Plevová, P Ovesná, L Radová, M Doubek, Š Pavlová, Š Pospíšilov, V Bryja
    Haematologica, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use
    Authors: JMJ Van den Be, ELJM Smits, M Versteven, H De Reu, ZN Berneman, VFI Van Tendel, E Lion
    J Immunol Res, 2017;2017(0):1975902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. CCR7 mediates human breast cancer cell invasion, migration by inducing epithelial-mesenchymal transition and suppressing apoptosis through AKT pathway
    Authors: B Xu, M Zhou, W Qiu, J Ye, Q Feng
    Cancer Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Dengue virus infection induces interferon-lambda1 to facilitate cell migration
    Sci Rep, 2016;6(0):24530.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes
    Authors: RB Jones, S Mueller, R O'Connor, K Rimpel, DD Sloan, D Karel, HC Wong, EK Jeng, AS Thomas, JB Whitney, SY Lim, C Kovacs, E Benko, S Karandish, SH Huang, MJ Buzon, M Lichterfel, A Irrinki, JP Murry, A Tsai, H Yu, R Geleziunas, A Trocha, MA Ostrowski, Irvi
    PLoS Pathog, 2016;12(4):e1005545.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Autocrine Signaling by Wnt-5a Deregulates Chemotaxis of Leukemic Cells and Predicts Clinical Outcome in Chronic Lymphocytic Leukemia.
    Authors: Janovska P, Poppova L, Plevova K, Plesingerova H, Behal M, Kaucka M, Ovesna P, Hlozkova M, Borsky M, Stehlikova O, Brychtova Y, Doubek M, Machalova M, Baskar S, Kozubik A, Pospisilova S, Pavlova S, Bryja V
    Clin Cancer Res, 2016;22(2):459-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Human breast cancer-derived soluble factors facilitate CCL19-induced chemotaxis of human dendritic cells
    Sci Rep, 2016;6(0):30207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Asymmetry of VANGL2 in migrating lymphocytes as a tool to monitor activity of the mammalian WNT/planar cell polarity pathway.
    Authors: Kaucka M, Petersen J, Janovska P, Radaszkiewicz T, Smyckova L, Daulat A, Borg J, Schulte G, Bryja V
    Cell Commun Signal, 2015;13(0):2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Selective susceptibility of human skin antigen presenting cells to productive dengue virus infection.
    Authors: Cerny D, Haniffa M, Shin A, Bigliardi P, Tan B, Lee B, Poidinger M, Tan E, Ginhoux F, Fink K
    PLoS Pathog, 2014;10(12):e1004548.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Tiam1/Rac1 signals contribute to the proliferation and chemoresistance, but not motility, of chronic lymphocytic leukemia cells.
    Authors: Hofbauer, Sebastia, Krenn, Peter W, Ganghammer, Sylvia, Asslaber, Daniela, Pichler, Ulrike, Oberascher, Karin, Henschler, Reinhard, Wallner, Michael, Kerschbaum, Hubert, Greil, Richard, Hartmann, Tanja N
    Blood, 2014;123(14):2181-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Vector-encoded Helicobacter pylori neutrophil-activating protein promotes maturation of dendritic cells with Th1 polarization and improved migration.
    Authors: Ramachandran M, Jin C, Yu D, Eriksson F, Essand M
    J Immunol, 2014;193(5):2287-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. An in-depth comparison of latent HIV-1 reactivation in multiple cell model systems and resting CD4+ T cells from aviremic patients.
    Authors: Spina, Celsa A, Anderson, Jenny, Archin, Nancie M, Bosque, Alberto, Chan, Jonathan, Famiglietti, Marylind, Greene, Warner C, Kashuba, Angela, Lewin, Sharon R, Margolis, David M, Mau, Matthew, Ruelas, Debbie, Saleh, Suha, Shirakawa, Kotaro, Siliciano, Robert F, Singhania, Akul, Soto, Paula C, Terry, Valeri H, Verdin, Eric, Woelk, Christop, Wooden, Stacey, Xing, Sifei, Planelles, Vicente
    PLoS Pathog, 2013;9(12):e1003834.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation.
    Authors: Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M
    Eur. J. Immunol., 2012;42(4):936-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.
    Authors: Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M
    J Transl Med, 2011;9(0):151.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
    Authors: Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
    Ann. Neurol., 2009;66(4):521-31.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  20. Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication.
    Authors: Damas JK, Landro L, Fevang B, Heggelund L, Tjonnfjord GE, Floisand Y, Halvorsen B, Froland SS, Aukrust P
    Clin. Exp. Immunol., 2009;157(3):400-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.
    Authors: Lim HW, Kim CH
    J. Immunol., 2007;179(11):7448-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  25. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Expression of CCX CKR in pulmonary sarcoidosis.
    Authors: Kriegova E, Tsyrulnyk A, Arakelyan A, Mrazek F, Ordeltova M, Petzmann S, Zatloukal J, Kolek V, du Bois RM, Popper H, Petrek M
    Inflamm. Res., 2006;55(10):441-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.
    Authors: Curiel, Tyler J, Coukos, George, Zou, Linhua, Alvarez, Xavier, Cheng, Pui, Mottram, Peter, Evdemon-Hogan, Melina, Conejo-Garcia, Jose R, Zhang, Lin, Burow, Matthew, Zhu, Yun, Wei, Shuang, Kryczek, Ilona, Daniel, Ben, Gordon, Alan, Myers, Leann, Lackner, Andrew, Disis, Mary L, Knutson, Keith L, Chen, Lieping, Zou, Weiping
    Nat Med, 2004;10(9):942-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004;78(16):8720-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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