Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera) Protein

Name: Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera) Protein
Brand: R&D Systems
SKU: 979-MC
Price: 257 USD
Availability: InStock

R&D Systems | Catalog # 979-MC

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems NS0-derived Recombinant Human CCL2/MCP-1 (Mucin Stalk Chimera) Protein (979-MC)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P13500 (CCL2) & P78423 (CX3CL1 Mucin-like Stalk)

Applications

Bioactivity

View all CCL2/JE/MCP-1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CCL2/JE/MCP-1 protein

Human CCL2 (Gln24 - Thr99) Accession # P13500	GS	Human Fractalkine mucin-like stalk (Phe103 - Gln341) Accession # P78423	KQN	6-His tag
N-terminus				C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln24 predicted

Predicted Molecular Mass

35 kDa

SDS-PAGE

70-95 kDa, reducing conditions

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2.
The ED₅₀ for this effect is 0.20-0.60 µg/mL.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 979-MC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 979-MC/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CCL2/JE/MCP-1

Fractalkine (also known as neurotactin and CX₃CL1) is one of two known transmembrane chemokines and is the only member of the CX₃C chemokine subfamily. Fractalkine is a type I membrane protein containing a chemokine domain tethered on a long mucin-like stalk that is connected to a transmembrane domain and a C-terminal intracellular domain. Expression of Fractalkine has been detected on endothelial cells, neurons, dendritic cells and a subset of mononuclear phagocytes. The expression of Fractalkine is upregulated in response to inflammatory signals. Fractalkine binds with high affinity to CX₃CR1 and mediates the tight binding of leukocytes expressing CX₃CR1 independently of G protein activation under flow conditions. In the MCP-1/mucin-like stalk chimera, the chemokine domain of human Fractalkine is replaced by human MCP-1 (CCL2), a ligand for CCR2.