Recombinant Human CCL2/MCP-1 Protein

Formulations:
Catalog # Availability Size / Price Qty
279-MC-010
279-MC-050
Recombinant Human CCL2/MCP-1 Protein Data
3 Images
Product Details
Citations (67)
FAQs
Reviews (4)

Recombinant Human CCL2/MCP-1 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 5‑30 ng/mL.
Source
E. coli-derived human CCL2/JE/MCP-1 protein
Gln24-Thr99
Accession #
N-terminal Sequence
Analysis
Gln24
Predicted Molecular Mass
8.7 kDa
SDS-PAGE
10 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

279-MC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

279-MC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED50for this effect is 5-30 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human CCL2/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 10 kDa.

Mass Spectrometry View Larger

MALDI-TOF analysis of Recombinant Human CCL2/MCP-1. The major peak corresponds to the calculated molecular mass, 8679 Da. The minor peak at 8887 is a matrix-associated artifact of the MALDI-TOF.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2, also called monocyte chemotactic protein‑1 (MCP‑1) or JE, is a member of the C‑C or beta  chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid‑modified glutamine, occurs naturally by metalloproteinase cleavage and down‑regulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7‑13.5 kDa on SDS‑PAGE due to non‑covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78‑79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2‑mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7).

References
  1. Yoshimura, T. et al. (1989) FEBS Lett. 244:487.
  2. Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
  3. Proost, P. et al.(1998) J. Immunol. 160:4034.
  4. Ju Lee, H. et al. (2015) J. Immunol. 194:3634.
  5. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
  6. Daly, C. et al. (2003) Microcirculation 10:247.
  7. Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.
    Entrez Gene IDs
    6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
    Alternate Names
    C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF

    Citations for Recombinant Human CCL2/MCP-1 Protein

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    67 Citations: Showing 1 - 10
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    1. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
      Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
      Nat Commun, 2020;11(1):2177.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Cell Culture
    2. EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands
      Authors: D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils
      J. Leukoc. Biol., 2020;0(0):.
      Species: Human
      Sample Types: Whole Cell
      Applications: Cell Culture
    3. PAK4 Kinase Activity Plays a Crucial Role in the Podosome Ring of Myeloid Cells
      Authors: E Foxall, A Staszowska, LM Hirvonen, M Georgouli, M Ciccioli, A Rimmer, L Williams, Y Calle, VS Moreno, S Cox, GE Jones, CM Wells
      Cell Rep, 2019;29(11):3385-3393.e6.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    4. Circulating levels of ATP is a biomarker of HIV cognitive impairment
      Authors: S Velasquez, L Prevedel, S Valdebenit, AM Gorska, M Golovko, N Khan, J Geiger, EA Eugenin
      EBioMedicine, 2019;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    5. CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma
      Authors: R Xu, Y Li, H Yan, E Zhang, X Huang, Q Chen, J Chen, J Qu, Y Liu, J He, Q Yi, Z Cai
      Cell Death Dis, 2019;10(10):781.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    6. Effects of GADL1 overexpression on cell migration and the associated morphological changes
      Authors: TN Wu, CK Chen, IC Liu, LS Wu, AT Cheng
      Sci Rep, 2019;9(1):5298.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    7. Estrogen Deficiency Promotes Cerebral Aneurysm Rupture by Upregulation of Th17 Cells and Interleukin-17A Which Downregulates E-Cadherin
      Authors: BL Hoh, K Rojas, L Lin, HZ Fazal, S Hourani, KW Nowicki, MB Schneider, K Hosaka
      J Am Heart Assoc, 2018;7(8):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    8. C-C motif chemokine ligand 2 induces proliferation and prevents lipopolysaccharide-induced inflammatory responses in bovine mammary epithelial cells
      Authors: C Yang, W Lim, H Bae, FW Bazer, G Song
      J. Dairy Sci., 2018;0(0):.
      Species: Bovine
      Sample Types: Whole Cells
      Applications: Bioassay
    9. Intermittent Hypoxia Enhances THP-1 Monocyte Adhesion and Chemotaxis and Promotes M1 Macrophage Polarization via RAGE
      Authors: J Zhou, W Bai, Q Liu, J Cui, W Zhang
      Biomed Res Int, 2018;2018(0):1650456.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    10. Long noncoding RNA LERFS negatively regulates rheumatoid synovial aggression and proliferation
      Authors: Y Zou, S Xu, Y Xiao, Q Qiu, M Shi, J Wang, L Liang, Z Zhan, X Yang, N Olsen, SG Zheng, H Xu
      J. Clin. Invest., 2018;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    11. Sulforaphane rescues amyloid-? peptide-mediated decrease in MerTK expression through its anti-inflammatory effect in human THP-1 macrophages
      Authors: KA Jhang, JS Park, HS Kim, YH Chong
      J Neuroinflammation, 2018;15(1):75.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    12. Antibody blockade of CLEC12A delays EAE onset and attenuates disease severity by impairing myeloid cell CNS infiltration and restoring positive immunity
      Authors: D Sagar, NP Singh, R Ginwala, X Huang, R Philip, M Nagarkatti, P Nagarkatti, K Neumann, J Ruland, AM Andrews, SH Ramirez, ZK Khan, P Jain
      Sci Rep, 2017;7(1):2707.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    13. Secreted Ectodomain of SIGLEC-9 and MCP-1 Synergistically Improve Acute Liver Failure in Rats by Altering Macrophage Polarity
      Authors: T Ito, M Ishigami, Y Matsushita, M Hirata, K Matsubara, T Ishikawa, H Hibi, M Ueda, Y Hirooka, H Goto, A Yamamoto
      Sci Rep, 2017;7(0):44043.
      Species: Rat
      Sample Types: In Vivo
      Applications: In Vivo
    14. miRNA-1246 induces pro-inflammatory responses in mesenchymal stem/stromal cells by regulating PKA and PP2A
      Authors: A Bott, N Erdem, S Lerrer, A Hotz-Wagen, C Breunig, K Abnaof, A Wörner, H Wilhelm, E Münsterman, A Ben-Baruch, S Wiemann
      Oncotarget, 2017;8(27):43897-43914.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    15. Decellularization and Solubilization of Porcine Liver for Use as a Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison
      Authors: RE Coronado, M Somaraki-C, S Natesan, RJ Christy, JL Ong, GA Halff
      Cell Transplant, 2017;26(12):1840-1854.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    16. Hyperglycemia-induced Renal P2X7 Receptor Activation Enhances Diabetes-related Injury
      Authors: RI Menzies, JWR Booth, JJ Mullins, MA Bailey, FWK Tam, JT Norman, RJ Unwin
      EBioMedicine, 2017;0(0):.
      Species: Human
      Sample Types: Cell Culture Supernates
      Applications: ELISA (Standard)
    17. Matrix degradability controls multicellularity of 3D cell migration
      Authors: B Trappmann, BM Baker, WJ Polacheck, CK Choi, JA Burdick, CS Chen
      Nat Commun, 2017;8(1):371.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    18. Aberrant SYK kinase signaling is essential for tumorigenesis induced by TSC2 inactivation
      Authors: Y Cui, WK Steagall, A Lamattina, G Pacheco-Ro, M Stylianou, P Kidambi, B Stump, F Golzarri, IO Rosas, C Priolo, EP Henske, J Moss, S El-Chemaly
      Cancer Res, 2017;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    19. p115 RhoGEF activates the Rac1 GTPase signaling cascade in MCP1-induced vascular smooth muscle cell migration and proliferation
      Authors: NK Singh, J Janjanam, GN Rao
      J. Biol. Chem., 2017;0(0):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    20. Macrophages Enhance Migration in Inflammatory Breast Cancer Cells via RhoC GTPase Signaling
      Sci Rep, 2016;6(0):39190.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    21. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
      Authors: Benjamin Malard
      Artif Organs, 2016;0(0):.
      Species: Human
      Sample Types: Plasma
      Applications: Hemofiltration Procedure
    22. Serum chemokine (CC motif) ligand 2 level as a diagnostic, predictive, and prognostic biomarker for prostate cancer.
      Authors: Kouji Izumi, Atsushi Mizokami, Hsiu-Ping Lin, Hui-Min Ho, Hiroaki Iwamoto, Aerken Maolake, Ariunbold Natsagdor, Yasuhide Kitagawa, Yoshifumi Kadono, Hiroshi Miyamoto, Chiung-Kuei Huang, Mikio Namiki, Wen-Jye Lin
      Oncotarget, 2016;0(0):1949-2553.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    23. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
      Cell Death Dis, 2016;7(12):e2565.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    24. PARP inhibition in leukocytes diminishes inflammation via effects on integrins/cytoskeleton and protects the blood-brain barrier
      J Neuroinflammation, 2016;13(1):254.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    25. Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression
      Authors: RG de Bruin, L Shiue, J Prins, HC de Boer, A Singh, WS Fagg, JM van Gils, JM Duijs, S Katzman, AO Kraaijevel, S Böhringer, WY Leung, SM Kielbasa, JP Donahue, PH van der Za, R Sijbom, CM van Alem, I Bot, C van Kooten, JW Jukema, H Van Esch, T Rabelink
      Nat Commun, 2016;7(0):10846.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    26. The Effect of MCP-1/CCR2 on the Proliferation and Senescence of Epidermal Constituent Cells in Solar Lentigo
      Authors: Woo Jin Lee
      Int J Mol Sci, 2016;17(6):.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    27. Cytokine-Like 1 Regulates Cardiac Fibrosis via Modulation of TGF-? Signaling
      PLoS ONE, 2016;11(11):e0166480.
      Species: Rat
      Sample Types: Whole Cells
      Applications: Bioassay
    28. Inhibition of T-cell activation by retinal pigment epithelial cells derived from induced pluripotent stem cells.
      Authors: Sugita S, Kamao H, Iwasaki Y, Okamoto S, Hashiguchi T, Iseki K, Hayashi N, Mandai M, Takahashi M
      Invest Ophthalmol Vis Sci, 2015;56(2):1051-62.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    29. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival.
      Authors: Kayisli U, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson H, Shapiro J, Summerfield T, Huang S, Prelle K, Schatz F, Lockwood C
      Proc Natl Acad Sci U S A, 2015;112(16):5153-8.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    30. Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells.
      Authors: Lee H, Ko J, Jeong H, Ko A, Kim M, Wee W, Yoon S, Oh J
      J Immunol, 2015;194(8):3634-45.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay
    31. S100A9 induced inflammatory responses are mediated by distinct damage associated molecular patterns (DAMP) receptors in vitro and in vivo.
      Authors: Chen, Bo, Miller, Allison, Rebelatto, Marlon, Brewah, Yambasu, Rowe, Daniel C, Clarke, Lori, Czapiga, Meggan, Rosenthal, Kim, Imamichi, Tomozumi, Chen, Yan, Chang, Chew-Shu, Chowdhury, Partha S, Naiman, Brian, Wang, Yue, Yang, De, Humbles, Alison A, Herbst, Ronald, Sims, Gary P
      PLoS ONE, 2015;10(2):e0115828.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    32. Technical advance: live-imaging analysis of human dendritic cell migrating behavior under the influence of immune-stimulating reagents in an organotypic model of lung.
      Authors: Nguyen Hoang A, Chen P, Bjornfot S, Hogstrand K, Lock J, Grandien A, Coles M, Svensson M
      J Leukoc Biol, 2014;96(3):481-9.
    33. Immunogenic calreticulin exposure occurs through a phylogenetically conserved stress pathway involving the chemokine CXCL8.
      Authors: Sukkurwala A, Martins I, Wang Y, Schlemmer F, Ruckenstuhl C, Durchschlag M, Michaud M, Senovilla L, Sistigu A, Ma Y, Vacchelli E, Sulpice E, Gidrol X, Zitvogel L, Madeo F, Galluzzi L, Kepp O, Kroemer G
      Cell Death Differ, 2014;21(1):59-68.
    34. CD14(hi)HLA-DR(dim) macrophages, with a resemblance to classical blood monocytes, dominate inflamed mucosa in Crohn's disease.
      Authors: Thiesen S, Janciauskiene S, Uronen-Hansson H, Agace W, Hogerkorp C, Spee P, Hakansson K, Grip O
      J Leukoc Biol, 2014;95(3):531-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    35. Transcriptomic profiling of the development of the inflammatory response in human monocytes in vitro.
      Authors: Italiani, Paola, Mazza, Emilia M, Lucchesi, Davide, Cifola, Ingrid, Gemelli, Claudia, Grande, Alexis, Battaglia, Cristina, Bicciato, Silvio, Boraschi, Diana
      PLoS ONE, 2014;9(2):e87680.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    36. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
      Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
      J Immunol, 2014;192(10):4674-84.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    37. Chemokine-coupled beta2 integrin-induced macrophage Rac2-Myosin IIA interaction regulates VEGF-A mRNA stability and arteriogenesis.
      Authors: Morrison A, Yarovinsky T, Young B, Moraes F, Ross T, Ceneri N, Zhang J, Zhuang Z, Sinusas A, Pardi R, Schwartz M, Simons M, Bender J
      J Exp Med, 2014;211(10):1957-68.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    38. The novel role of IL-7 ligation to IL-7 receptor in myeloid cells of rheumatoid arthritis and collagen-induced arthritis.
      Authors: Chen Z, Kim S, Chamberlain N, Pickens S, Volin M, Volkov S, Arami S, Christman J, Prabhakar B, Swedler W, Mehta A, Sweiss N, Shahrara S
      J Immunol, 2013;190(10):5256-66.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    39. Novel role for p21-activated kinase 2 in thrombin-induced monocyte migration.
      Authors: Gadepalli R, Kotla S, Heckle M, Verma S, Singh N, Rao G
      J Biol Chem, 2013;288(43):30815-31.
    40. Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.
      Authors: Kundumani-Sridharan, Venkates, Singh, Nikhlesh, Kumar, Sanjay, Gadepalli, Ravisekh, Rao, Gadipart
      J Biol Chem, 2013;288(30):22150-62.
      Species: Human
      Sample Types: Whole Cells
      Applications: Cell Culture
    41. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
      Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
      Arterioscler Thromb Vasc Biol, 2013;33(5):911-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    42. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
      Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
      Nature, 2012;487(7408):505-9.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    43. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
      Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
      J. Biol. Chem., 2011;286(2):961-75.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    44. A multiplex immunoassay for human adipokine profiling.
      Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
      Clin. Chem., 2010;56(0):1320.
    45. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
      Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
      J. Immunol., 2010;184(3):1642-52.
      Species: Human
      Sample Types: Carbohydrates
      Applications: Bioassay
    46. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
      Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
      Immunology, 2010;129(4):536-46.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    47. IL-17 induces monocyte migration in rheumatoid arthritis.
      Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
      J. Immunol., 2009;182(6):3884-91.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    48. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
      Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
      J. Leukoc. Biol., 2008;84(4):1109-19.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    49. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
      Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
      Blood, 2008;112(3):493-503.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    50. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
      Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
      J. Immunol., 2008;181(11):8053-67.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    51. Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells.
      Authors: Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC
      J. Immunol., 2007;179(5):3332-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    52. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
      Authors: Pfleger C, Schloot N, ter Veld F
      J. Immunol. Methods, 2007;329(1):214-8.
      Species: N/A
      Sample Types: N/A
      Applications: ELISA (Standard)
    53. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
      Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
      Clin. Chem., 2007;53(11):2010-2.
      Species: N/A
      Sample Types: N/A
      Applications: ELISA (Standard)
    54. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
      Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
      Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    55. Ultrasensitive flow-based immunoassays using single-molecule counting.
      Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
      Clin. Chem., 2007;53(11):1990-5.
      Species: N/A
      Sample Types: N/A
      Applications: ELISA (Standard)
    56. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
      Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
      Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    57. The monocyte chemoattractant protein-1/cognate CC chemokine receptor 2 system affects cell motility in cultured human podocytes.
      Authors: Burt D, Salvidio G, Tarabra E, Barutta F, Pinach S, Dentelli P, Camussi G, Perin PC, Gruden G
      Am. J. Pathol., 2007;171(6):1789-99.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    58. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
      Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
      Exp. Cell Res., 2006;312(10):1909-23.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    59. Novel antiviral activity of chemokines.
      Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
      Virology, 2006;350(2):484-92.
      Species: Virus
      Sample Types: Virus
      Applications: Binding Assay
    60. CD16+ monocyte-derived macrophages activate resting T cells for HIV infection by producing CCR3 and CCR4 ligands.
      Authors: Ancuta P, Autissier P, Wurcel A, Zaman T, Stone D, Gabuzda D
      J. Immunol., 2006;176(10):5760-71.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    61. HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP-1)-dependent mechanism: possible role in NeuroAIDS.
      Authors: Eugenin EA, Dyer G, Calderon TM, Berman JW
      Glia, 2005;49(4):501-10.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    62. trans fatty acids and systemic inflammation in heart failure.
      Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
      Am. J. Clin. Nutr., 2004;80(6):1521-5.
      Species: N/A
      Sample Types: N/A
      Applications: ELISA (Standard)
    63. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
      Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
      J. Immunol., 2004;173(11):6592-602.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    64. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
      Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
      Blood, 2004;104(2):314-20.
      Species: N/A
      Sample Types: N/A
      Applications: ELISA (Standard)
    65. Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
      Authors: Traves SL, Smith SJ, Barnes PJ, Donnelly LE
      J. Leukoc. Biol., 2004;76(2):441-50.
      Species: Human
      Sample Types: Whole Cells
      Applications: Bioassay
    66. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
      Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
      J. Neurochem., 2002;81(2):257-69.
      Species: Rat
      Sample Types: Whole Tissue
      Applications: Binding Assay
    67. Enhanced tumor trafficking of GD2 chimeric antigen receptor T cells by expression of the chemokine receptor CCR2b.
      Authors: Craddock J, Lu A, Bear A, Pule M, Brenner M, Rooney C, Foster A
      J Immunother, 0;33(8):780-8.
      Species: Human
      Sample Types: Whole Cells
      Applications: CAR-T (Bioassay)

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    Reviews for Recombinant Human CCL2/MCP-1 Protein

    Average Rating: 5 (Based on 4 Reviews)

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    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 07/08/2019
    Application: In vitro bioactivity in cell culture

    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 11/26/2018
    Application: Immunoassay Standard

    The recombinant human CCL2 protein was used as the calibration reagent in MSD assay with biotinylated MAB279 (capture) and Sulfo-Tag labeled AF-279-NA (detection) antibodies.


    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 02/05/2018
    Application: Immunoassay Standard

    Recombinant Human CCL2/MCP-1 Protein
    By Anonymous on 06/13/2017
    Application: Cell migration/motility
    Reason for Rating: I have been using this protein for cell migration and cell signaling assays and it works very well.

    MCP1-induced smooth muscle cells migration using wound closure assay. I use at 50 ng/ml concentration in serum-free media.