Recombinant Human CCL25/TECK Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR9. The ED50 for this effect is 30-180 ng/mL.
  • Source
    E. coli-derived Gln24-Leu150
  • Accession #
  • N-terminal Sequence
    Analysis
    Gln24
  • Predicted Molecular Mass
    14 kDa
  • SDS-PAGE
    14 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9046-TK/CF
 
9046-TK
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Binding Activity
Recombinant Human CCL25/TECK (Catalog #
9046-TK/CF) chemoattracts the BaF3 pro-B cells transfected with human CCR9. The ED50 for this effect is 30-180 ng/mL.
Background: CCL25/TECK
CCL25, also known as TECK (thymus-expressed chemokine), is a CC chemokine that regulates the trafficking of lymphocytes in the thymus and small intestine. Mature human CCL25 shares 40% amino acid sequence identity with mouse and rat CCL25 (1). CCL25 is produced by stromal cells in the thymus and epithelial cells of the small intestine, particularly the jejunum and ileum (1-3). It binds to and induces chemoattraction through CCR9 (1, 4, 5), and both human and mouse proteins act on human CCR9 (4). CCR9 is expressed on immature pre-T cells and thymocytes (5, 6). CCL25 induces the homing of several lymphocyte populations to the small intestine (3), including Integrin alpha 4 beta 7+ gamma δ T cells (6, 7), Integrin alpha E beta 7+ CD8+ T cells (8), and IgA-producing plasma cells (2). In cancer, functional CCR9 mediates the metastasis of melanoma cells to the small intestine (9), contributes to the CCL25-dependent migration and invasion of some breast carcinomas (10), and attracts mesenchymal stromal cells to CCL25-expressing multiple myelomas (11). CCL25 contributes to the severity of chronic inflammation in rheumatoid arthritis where it attracts CCR9+ monocytes and macrophages (12), in endometriosis where it promotes the invasiveness of stromal cells (13), and in atherosclerosis where it contributes to the accumulation of CCR9+ macrophages in arterial plaques (14).
  • References:
    1. Vicari, A.P. et al. (1997) Immunity 7:291.
    2. Bowman, E.P. et al. (2002) J. Exp. Med. 195:269.
    3. Kunkel, E.J. et al. (2000) J. Exp. Med. 192:761.
    4. Zaballos, A. et al. (1999) J. Immunol. 162:5671.
    5. Youn, B.-S. et al. (1999) Blood 94:2533.
    6. Uehara, S. et al. (2002) J. Immunol. 168:134.
    7. Costa, M.F.S. et al. (2012) Eur. J. Immunol. 42:1250.
    8. Svensson, M. et al. (2002) J. Clin. Invest. 110:1113.
    9. Amersi, F.F. et al. (2008) Clin. Cancer Res. 14:638.
    10. Johnson-Holiday, C. et al. (2011) Int. J. Oncol. 38:1279.
    11. Xu, S. et al. (2012) Stem Cells 30:266.
    12. Schmutz, C. et al. (2010) Arthritis Res. Ther. 12:R161.
    13. Wang, Y. et al. (2010) Cell. Mol. Immunol. 7:51.
    14. Alla, J.A. et al. (2010) J. Biol. Chem. 285:23496.
  • Entrez Gene IDs:
    6370 (Human); 20300 (Mouse)
  • Alternate Names:
    C-C motif chemokine 25; CCL25; chemokine (C-C motif) ligand 25; Chemokine TECK; Ck beta-15; Ckb15; SCYA25TECKvar; small inducible cytokine subfamily A (Cys-Cys), member 25; Small-inducible cytokine A25; TECK; TECKMGC150327; thymus expressed chemokine; Thymus-expressed chemokine
Related Research Areas

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