Recombinant Human CCL26/Eotaxin-3 Protein

R&D Systems | Catalog # 653-E3

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CCL26/Eotaxin-3 Protein (653-E3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q9Y258

Applications

Bioactivity
View all CCL26/Eotaxin-3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CCL26/Eotaxin-3 protein
Ser27-Leu94

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

8.2 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3.
The ED50 for this effect is 0.3‑1.5 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 653-E3 in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 653-E3
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 653-E3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL26/Eotaxin-3

Eotaxin-3, also named CCL26 or SCYA26, is a novel human CC chemokine that maps to chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3/CCL26 has been shown to be constitutively expressed in the heart and ovary. In addition, low levels of Eotaxin-3/CCL26 expression can also be detected in various tissues. The expression of Eotaxin-3/CCL26 in vascular endothelial cells has been shown to be up-regulated by IL-13 and IL-4.

Eotaxin-3/CCL26 cDNA encodes a 94 amino acid (aa) residue protein with a putative signal peptide of either 23 or 26 aa residues. RecombinantEotaxin-3/CCL26 has been produced in insect cells using a baculovirus expression system and shown to contain 71 aa residues. RecombinantEotaxin-3/CCL26 is chemotactic for eosinophils and PHA-activated T cells. Eotaxin-3/CCL26 induces calcium flux in eosinophils as well as in CCR3-transfected cells. Eotaxin-3/CCL26 has also been shown to cross-desensitize cells to other CCR3 ligands. Both the 71 aa residue and 68 aa residue variants of recombinant Eotaxin-3 have been expressed in E. coli and found to have equal potency in inducing chemotaxis of a human CCR3-transfected cell line.

References

  1. Gou, R-F. et al. (1999) Genomics 58:313.
  2. Kitamura, M. et al. (1999) J. Biol. Chem. 274:27975.
  3. Shinkai, A. et al. (1999) J. Immunol. 163:1602.

Alternate Names

Eotaxin-3, Eotaxin3, SCYA26

Entrez Gene IDs

10344 (Human)

Gene Symbol

CCL26

UniProt

Q9Y258

Additional CCL26/Eotaxin-3 Products

Product Documents for Recombinant Human CCL26/Eotaxin-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 653-E3 (with carrier)
Download SDS 653-E3/CF (carrier free)

Product Specific Notices for Recombinant Human CCL26/Eotaxin-3 Protein

For research use only

Citations for Recombinant Human CCL26/Eotaxin-3 Protein

Customer Reviews for Recombinant Human CCL26/Eotaxin-3 Protein (1)

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    Recombinant Human CCL26/Eotaxin-3 Protein 653-E3

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