CCL27, also known as CTACK (cutaneous T cell-attracting chemokine), ALP, ILC, and ESkine, is a member of the CC family of chemokines (1). Mature human CCL27 is an 88 amino acid (aa) protein that shares 57% aa sequence identity with mouse and rat CCL27 (2). It shares 11% - 35% aa sequence identity with other human CC chemokines. An alternately spliced form of mouse CCL27, known as PESKY, is localized to the nucleus and promotes cellular migration (3). CCL27 is constitutively expressed by keratinocytes and is upregulated by inflammatory stimuli and in wounded skin (4 - 7). CCL27 binds the chemokine receptor CCR10, glycosaminoglycans in the extracellular matrix, sulfated tyrosine residues on PSGL-1, and determinants on the surface of fibroblasts and endothelial cells (5, 7 - 9). CCL27 cooperates with CCL17/TARC in inducing the migration of cutaneous lymphocyte antigen (CLA) positive memory T cells to the skin during inflammation (4, 6, 10 - 12). Endothelial cell-bound CCL27 can mediate the adhesion of those cells to CLA+ T cells (6). CCL27 also induces the migration of keratinocyte precursors from bone marrow to the skin, thereby promoting wound healing (7). In humans, serum CCL27 levels are elevated and correlate with disease severity in atopic dermatitis, psoriasis vulgaris, and mycosis fungoides (13 - 15).
Recombinant Human CCL27/CTACK Protein
R&D Systems | Catalog # 376-CT
Key Product Details
- R&D Systems E. coli-derived Recombinant Human CCL27/CTACK Protein (376-CT)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Phe25-Gly112
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 0.1‑0.4 µg/mL.
Reviewed Applications
Read 2 reviews rated 5 using 376-CT in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 376-CT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 376-CT/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CCL27/CTACK
References
- Kunkel, L. and E.C. Butcher (2002) Immunity 16:1.
- Ishikawa-Mochizuki, I. et al. (1999) FEBS Lett. 460:544.
- Gortz, A. et al. (2002) J. Immunol. 169:1387.
- Morales, J. et al. (1999) Proc. Natl. Acad. Sci. 96:14470.
- Homey, B. et al. (2000) J. Immunol. 164:3465.
- Homey, B. et al. (2002) Nat. Med. 8:157.
- Inokuma, D. et al. (2006) Stem Cells 24:2810.
- Jarmin, D. et al. (2000) J. Immunol. 164:3460.
- Hirata, T. et al. (2004) J. Biol. Chem. 279:51775.
- Vestergaard, C. et al. (2004) Exp. Dermatol. 13:551.
- Reiss, Y. et al. (2001) J. Exp. Med. 194:1541.
- Soler, D. et al. (2003) Blood 101:1677.
- Kakinuma, T. et al. (2003) J. Allergy Clin. Immunol. 111:592.
- Hijnen, D. et al. (2004) J. Allergy Clin. Immunol. 113:334.
- Fujita, Y. et al. (2006) Clin. Cancer Res. 12:2670.
Alternate Names
Gene Symbol
UniProt
Additional CCL27/CTACK Products
Product Documents for Recombinant Human CCL27/CTACK Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human CCL27/CTACK Protein
For research use only
Related Research Areas
Citations for Recombinant Human CCL27/CTACK Protein
Powered by Bioz
Customer Reviews for Recombinant Human CCL27/CTACK Protein (2)
Have you used Recombinant Human CCL27/CTACK Protein?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
-
Application: Immunoassay StandardVerified Customer | Posted 09/05/2019
-
Application: Immunoassay StandardVerified Customer | Posted 11/15/2017MAB376 was used in a sandwich ELISA with biotinylated AF376 as detection at a concentration of 50ng/ml. This recombinant protein was used as the calibrator.
There are no reviews that match your criteria.
Associated Pathways
