Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.7-3.5 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Human CD2F-10/SLAMF9
    (Arg19-Ser210)
    Accession # Q96A28
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg19 & Val33
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    48 kDa (monomer)
  • SDS-PAGE
    60-70 kDa, reducing conditions
8235-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
1 μg/lane of Recombinant Human CD2F/SLAMF9 Fc Chimera (Catalog # 8235-CD) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing R bands at 60-70 kDa and NR bandsat 150-160 kDa.
Background: CD2F-10/SLAMF9

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.

  • References:
    1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
    2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
    3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
    4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
  • Long Name:
    CD2 Family 10/SLAM Family Member 9
  • Entrez Gene IDs:
    89886 (Human); 98365 (Mouse)
  • Alternate Names:
    CD2 family member 10; CD2F10; CD2F-10; CD2F-10MGC88194; CD84 homolog 1; CD84H1; CD84-H1; CD84-H1cluster of differentiation 2 antigen family member 10; cluster of differentiation 84 homolog 1; SF2001; signaling lymphocytic activation molecule family member 2001; signaling lymphocytic activation molecule family member 9; SLAM family member 9; SLAMF9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016;7(0):11473.
    Species: Human
    Sample Type: Recombinant Protein
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