Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
8235-CD-050
Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.7-3.5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD2F-10/SLAMF9 protein
Human CD2F-10/SLAMF9
(Arg19-Ser210)
Accession # Q96A28
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Arg19 & Val33
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
48 kDa (monomer)
SDS-PAGE
60-70 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8235-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

1 μg/lane of Recombinant Human CD2F/SLAMF9 Fc Chimera (Catalog # 8235-CD) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing R bands at 60-70 kDa and NR bandsat 150-160 kDa.

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Background: CD2F-10/SLAMF9

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.

References
  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
  2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
  3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
  4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.
Long Name
CD2 Family 10/SLAM Family Member 9
Entrez Gene IDs
89886 (Human); 98365 (Mouse)
Alternate Names
CD2 family member 10; CD2F10; CD2F-10; CD2F-10MGC88194; CD84 homolog 1; CD84H1; CD84-H1; CD84-H1cluster of differentiation 2 antigen family member 10; cluster of differentiation 84 homolog 1; SF2001; signaling lymphocytic activation molecule family member 2001; signaling lymphocytic activation molecule family member 9; SLAM family member 9; SLAMF9

Citation for Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016;7(0):11473.
    Species: Human
    Sample Types: Recombinant Protein

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