Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF Summary
Accession # Q96A28
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
1 μg/lane of Recombinant Human CD2F/SLAMF9 Fc Chimera (Catalog # 8235-CD) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing R bands at 60-70 kDa and NR bandsat 150-160 kDa.
The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.
- Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.
- Fennelly, J.A. et al. (2001) Immunogenetics 53:599.
- Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.
- Fraser, C.C. et al. (2002) Immunogenetics 53:843.
Citation for Recombinant Human CD2F-10/SLAMF9 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
Nat Commun, 2016;7(0):11473.
Sample Types: Recombinant Protein
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