Recombinant Human CD300d Fc Chimera Protein, CF

R&D Systems | Catalog # 10273-CD

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD300d Fc Chimera Protein (10273-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q6UXZ3

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD300d Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD300d protein
Human CD300d
(Ala19-His165)
Accession # Q6UXZ3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

43 kDa

SDS-PAGE

50-70 kDa, under reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells.
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Human CD300d Fc Chimera Protein, CF

Recombinant Human CD300d Fc Chimera Protein Bioactivity

Recombinant Human CD300d Fc Chimera Protein Bioactivity

Recombinant Human CD300d Fc Chimera (Catalog # 10273-CD) inhibits anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 1‑6 μg/mL.
Recombinant Human CD300d Fc Chimera Protein SDS-PAGE

Recombinant Human CD300d Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD300d Fc Chimera (Catalog # 10273-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa and 100-140 kDa, respectively.

Formulation, Preparation, and Storage

10273-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD300d

CD300d (also known as CD300LD or CMRF35A4) is a member of the CD300 family of transmembrane glycoproteins belonging to the immunoregulatory signaling (IRS) family. CD300d, like most CD300 family members, is found exclusively on myeloid cells, including monocyte and granulocytes (1). CD300 members contain a V-type immunoglobulin-like domain with an additional pair of cysteine residues in the extracellular domain (ECD), a transmembrane region, and a short cytoplasmic tail (2, 3). The mature ECD of human CD300d is 146 amino acids (aa) and shares a 48% and 45% identity with mouse and rat CD300d, respectively. CD300d recruits ITAM-bearing Adaptor FceRg. CD300d interacts with all CD300 family members with exception of CD300c, and plays a role in the regulation and/or formation of CD300 complexes on the cell surface and consequently modulate the state of activation of myeloid cells. CD300 family members modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300d, along with CD300lf, has been identified as a receptor for permissive murine noroviral infection (MNoV) (4). Further, expression of murine CD300LF on human and other mammalian cell lines confers cross‑species permissively (5). Additional research into CD300 family member function during viral infections could help develop novel anti-viral therapies (6). Our in‑house data indicate that CD300D inhibits T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion.

References

  1. Comas-Casellas, E. et al. 2011. J. Biol. Chem. 287:9682.
  2. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
  3. Borrego F. (2013) Blood 121:1951.
  4. Haga, K. et al. 2016. PNAS. 113:E6248.
  5. Orchard, R. et al. 2016. Science. 353:933.
  6. Graziano, V et al. 2019. Viruses. 11:pii.

Long Name

CD300 Molecule-Like Family Member D

Alternate Names

CD300d, CD300LD, CLM-5, MAIR-IV

Entrez Gene IDs

100131439 (Human); 217305 (Mouse); 100361633 (Rat); 100686711 (Canine)

Gene Symbol

CD300LD

UniProt

Q6UXZ3

Additional CD300d Products

Product Documents for Recombinant Human CD300d Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CD300d Fc Chimera Protein, CF

For research use only

Customer Reviews for Recombinant Human CD300d Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CD300d Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Human CD300d Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes