Recombinant Human CD300d Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10273-CD-050
Recombinant Human CD300d Fc Chimera Protein, CF Bioactivity
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Recombinant Human CD300d Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD300d protein
Human CD300d
(Ala19-His165)
Accession # Q6UXZ3
IEGRMDHuman IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ala19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa
SDS-PAGE
50-70 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10273-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human CD300d Fc Chimera (Catalog # 10273-CD) inhibits anti-CD3 antibody induced IL-2 secretion by human T cells. The ED50 for this effect is 1‑6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD300d Fc Chimera (Catalog # 10273-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa and 100-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300d

CD300d (also known as CD300LD or CMRF35A4) is a member of the CD300 family of transmembrane glycoproteins belonging to the immunoregulatory signaling (IRS) family. CD300d, like most CD300 family members, is found exclusively on myeloid cells, including monocyte and granulocytes (1). CD300 members contain a V-type immunoglobulin-like domain with an additional pair of cysteine residues in the extracellular domain (ECD), a transmembrane region, and a short cytoplasmic tail (2, 3). The mature ECD of human CD300d is 146 amino acids (aa) and shares a 48% and 45% identity with mouse and rat CD300d, respectively. CD300d recruits ITAM-bearing Adaptor FceRg. CD300d interacts with all CD300 family members with exception of CD300c, and plays a role in the regulation and/or formation of CD300 complexes on the cell surface and consequently modulate the state of activation of myeloid cells. CD300 family members modulate a broad and diverse array of immune cell processes via their paired activating and inhibitory receptor functions (2, 3). Mouse CD300d, along with CD300lf, has been identified as a receptor for permissive murine noroviral infection (MNoV) (4). Further, expression of murine CD300LF on human and other mammalian cell lines confers cross‑species permissively (5). Additional research into CD300 family member function during viral infections could help develop novel anti-viral therapies (6). Our in‑house data indicate that CD300D inhibits T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion.

References
  1. Comas-Casellas, E. et al. 2011. J. Biol. Chem. 287:9682.
  2. Clark, G.J. et al. (2009) Trends in Immunology 30:209.
  3. Borrego F. (2013) Blood 121:1951.
  4. Haga, K. et al. 2016. PNAS. 113:E6248.
  5. Orchard, R. et al. 2016. Science. 353:933.
  6. Graziano, V et al. 2019. Viruses. 11:pii.
Long Name
CD300 Molecule-Like Family Member D
Entrez Gene IDs
100131439 (Human); 217305 (Mouse); 100361633 (Rat); 100686711 (Canine)
Alternate Names
CD300 molecule-like family member d; CD300d; CD300LD; CLM-5; CMRF35-A4; immune receptor CD300d; MAIR-IV

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