Recombinant Human CD38 Protein, CF

R&D Systems | Catalog # 2404-AC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CD38 Protein (2404-AC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P28907

Applications

Enzyme Activity
View all CD38 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD38 protein
Val43-Ile300, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val43

Predicted Molecular Mass

31 kDa

SDS-PAGE

43 kDa, reducing conditions

Activity

Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 2404-AC in the following applications:

Scientific Data Images for Recombinant Human CD38 Protein, CF

Recombinant Human CD38 Protein Enzyme Activity.

Recombinant Human CD38 Protein (Catalog # 2404-AC) is measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose.

Formulation, Preparation, and Storage

2404-AC
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta  cells (4, 5).

References

  1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
  2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
  3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
  4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
  5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.

Long Name

Cluster of Differentiation 38

Alternate Names

ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Gene Symbol

CD38

UniProt

P28907

Additional CD38 Products

Product Documents for Recombinant Human CD38 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD38 Protein, CF

For research use only

Citations for Recombinant Human CD38 Protein, CF

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Protocols

View specific protocols for Recombinant Human CD38 Protein, CF (2404-AC):

Materials
  • Assay Buffer: 50 mM MES, pH 6.5
  • Recombinant Human CD38 (rhCD38) (Catalog # 2404-AC)
  • Substrate: NGD+ (Sigma, Catalog # N-5131), 10 mM stock in deionized water
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCD38 to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load in plate, 50 µL of 2 ng/µL rhCD38, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 300 nm and 410 nm (top read), respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard cyclic GDP ribose (cGDPR).

Per Well:
  • rhCD38: 0.1 µg
  • Substrate: 100 µM

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