Recombinant Human CD38 Protein, CF

Catalog # Availability Size / Price Qty
2404-AC-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
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Recombinant Human CD38 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260. The specific activity is >2,500 pmol/min/µg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived human CD38 protein
Val43-Ile300, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Val43
Predicted Molecular Mass
31 kDa
SDS-PAGE
43 kDa, reducing conditions

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Product Datasheets

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2404-AC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2404-AC

Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

Materials
  • Assay Buffer: 50 mM MES, pH 6.5
  • Recombinant Human CD38 (rhCD38) (Catalog # 2404-AC)
  • Substrate: NGD+ (Sigma, Catalog # N-5131), 10 mM stock in deionized water
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCD38 to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load in plate, 50 µL of 2 ng/µL rhCD38, and start the reaction by adding 50 µL of 200 µM Substrate. Include a Substrate Blank containing 50 µL Assay Buffer and 50 µL of 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 300 nm and 410 nm (top read), respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard cyclic GDP ribose (cGDPR).

Per Well:
  • rhCD38: 0.1 µg
  • Substrate: 100 µM
Reconstitution Calculator

Reconstitution Calculator

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Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta  cells (4, 5).

References
  1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
  2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
  3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
  4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
  5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.
Long Name
Cluster of Differentiation 38
Entrez Gene IDs
952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)
Alternate Names
2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase (EC:2.4.99.20); 2'-phospho-cyclic-ADP-ribose transferase; ADPRC 1; ADPRC1; ADP-ribosyl cyclase 1; ADP-ribosyl Cyclase; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase; cADPr hydrolase 1; CD_antigen: CD38; CD38 antigen (p45); CD38 antigen; CD38 molecule; CD38; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; Cyclic ADP-ribose Hydrolase; EC 2.4.99.20; EC 3.2.2.6; EC:3.2.2.6; NAD(+) nucleosidase; T10

Citations for Recombinant Human CD38 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. BST1 regulates nicotinamide riboside metabolism via its glycohydrolase and base-exchange activities
    Authors: K Yaku, S Palikhe, H Izumi, T Yoshida, K Hikosaka, F Hayat, M Karim, T Iqbal, Y Nitta, A Sato, ME Migaud, K Ishihara, H Mori, T Nakagawa
    Nature Communications, 2021;12(1):6767.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Diverse Functional Autoantibodies in Patients with COVID-19
    Authors: EY Wang, T Mao, J Klein, Y Dai, JD Huck, F Liu, NS Zheng, T Zhou, B Israelow, P Wong, C Lucas, J Silva, JE Oh, E Song, ES Perotti, S Fischer, M Campbell, JB Fournier, AL Wyllie, CBF Vogels, IM Ott, CC Kalinich, ME Petrone, AE Watkins, CD Cruz, SF Farhadian, WL Schulz, ND Grubaugh, AI Ko, A Iwasaki, AM Ring
    medRxiv : the preprint server for health sciences, 2020;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture

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