Recombinant Human CD39/ENTPD1 Protein, CF

Catalog # Availability Size / Price Qty
4397-EN-010
Product Details
Citations (2)
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Recombinant Human CD39/ENTPD1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996). The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD39/ENTPD1 protein
Thr38-Val478, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr38
Predicted Molecular Mass
51 kDa
SDS-PAGE
65-90 kDa, reducing conditions

Product Datasheets

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Background: CD39/ENTPD1

Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant human NTPDase-1 was expressed as a protein lacking its N- and C-terminal transmembrane domains, resulting in the secretion of the soluble recombinant human NTPDase-1 ectodomain. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). NTPDase-1 hydrolyzes the beta - and gamma  phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1 plays a role in the regulation of purinergic signaling (4). NTPDase-1 is involved in the processes of thromboregulation and vascular inflammation (5). The administration of soluble NTPDase-1 may have therapeutic applications for the treatment of some vascular and transplantation-associated diseases (6).

References
  1. Maliszewski, C.R. et al. (1994) J. Immunol. 153:3574.
  2. Rowe, M. et al. (1982) Int. J. Cancer 29:373.
  3. Kansas, G.S. et al. (1991) J. Immunol. 146:2235.
  4. Kishore, B.K. et al. (2005) Am. J. Physiol. Renal Physiol. 288:F1032.
  5. Marcus, A.J. et al. (2005) Semin. Thromb. Hemost. 31:234.
  6. Robson, S.C. et al. (2005) Semin. Thromb. Hemost. 31:217.
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 1
Entrez Gene IDs
953 (Human); 12495 (Mouse); 64519 (Rat); 102132860 (Cynomolgus Monkey)
Alternate Names
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

Citations for Recombinant Human CD39/ENTPD1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. ADPase CD39 Fused to Glycoprotein VI-Fc Boosts Local Antithrombotic Effects at Vascular Lesions
    Authors: H Degen, O Borst, M Ziegler, AK Mojica Mun, J Jamasbi, B Walker, S Göbel, J Fassbender, K Adler, R Brandl, G Münch, R Lorenz, W Siess, M Gawaz, M Ungerer
    J Am Heart Assoc, 2017;6(8):.
    Applications: EnzAct
  2. The ubiquitin ligase ASB4 promotes trophoblast differentiation through the degradation of ID2.
    Authors: Townley-Tilson, W H Davi, Wu, Yaxu, Ferguson, James E, Patterson, Cam
    PLoS ONE, 2014;9(2):e89451.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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