Malachite Green Phosphate Detection Kit

Catalog # Availability Size / Price Qty
Product Details
Citations (15)
Reviews (1)

Malachite Green Phosphate Detection Kit Summary

Assay Type
Malachite Green Phosphate Detection Kit
Assay Length
4 hours 40 minutes (after plate preparation)
Sample Volume Required
50 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

This Malachite Green Phosphate Detection Kit employs a simple, sensitive, reproducible, and non-radioactive method for measuring inorganic phosphate in aqueous solutions. The assay is based on the malachite green-molybdate binding reaction, and has a wide variety of applications, including the measurement of protein and lipid phosphorylation and phosphatase activity. The protocol presented is for the assay of phosphate in a 96-well microplate format. The Malachite Green Phosphate Detection Kit can also be run using 1 mL cuvettes or 384-well plates suitable for high-throughput screening.

Kit Content

  • Malachite Green Reagent A - Ammonium molybdate in 3M sulfuric acid
  • Malachite Green Reagent B - Malachite green oxalate and polyvinyl alcohol
  • Phosphate Standard

Other Reagents Required

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent


Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at room temperature. Do not use past expiration date.

Citations for Malachite Green Phosphate Detection Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. The effect of arginine-fluoride varnish on biochemical composition of multi-species biofilm
    Authors: MN Bijle, U Ashraf, MM Abdalla, CKY Yiu
    Journal of dentistry, 2021;108(0):103631.
    Species: Human
    Sample Types: Biofilm
  2. The C-terminal domain of feline and bovine SAMHD1 proteins has a crucial role in lentiviral restriction
    Authors: C Wang, K Zhang, L Meng, X Zhang, Y Song, Y Zhang, Y Gai, Y Zhang, B Yu, J Wu, S Wang, X Yu
    J. Biol. Chem., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
  3. Unfolding of a ClC chloride transporter retains memory of its evolutionary history
    Authors: D Min, RE Jefferson, Y Qi, JY Wang, MA Arbing, W Im, JU Bowie
    Nat. Chem. Biol., 2018;0(0):.
  4. Human alkaline phosphatase dephosphorylates microbial products and is elevated in preterm neonates with a history of late-onset sepsis
    Authors: M Pettengill, JD Matute, M Tresenrite, J Hibbert, D Burgner, P Richmond, J Luis Millá, A Ozonoff, T Strunk, A Currie, O Levy
    PLoS ONE, 2017;12(4):e0175936.
    Species: Human
    Sample Types: Recombinant Protein
  5. Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 Expression
    Authors: M Kumazoe, M Yamashita, Y Nakamura, K Takamatsu, J Bae, S Yamashita, S Yamada, H Onda, T Nojiri, K Kangawa, H Tachibana
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Tiamulin inhibits breast cancer growth and pulmonary metastasis by decreasing the activity of CD73
    Authors: X Yang, S Pei, H Wang, Y Jin, F Yu, B Zhou, H Zhang, D Zhang, D Lin
    BMC Cancer, 2017;17(1):255.
    Species: Human
    Sample Types: Whole Cells
  7. ADPase CD39 Fused to Glycoprotein VI-Fc Boosts Local Antithrombotic Effects at Vascular Lesions
    Authors: H Degen, O Borst, M Ziegler, AK Mojica Mun, J Jamasbi, B Walker, S Göbel, J Fassbender, K Adler, R Brandl, G Münch, R Lorenz, W Siess, M Gawaz, M Ungerer
    J Am Heart Assoc, 2017;6(8):.
    Species: Mouse
    Sample Types: Plasma
  8. CD73 promotes resistance to HER2/ErbB2 antibody therapy
    Authors: M Turcotte, D Allard, D Mittal, Y Bareche, L Buisseret, V Jose, S Pommey, V Delisle, S Loi, H Joensuu, PL Kellokumpu, C Sotiriou, MJ Smyth, J Stagg
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Bone marrow-derived mesenchymal stromal cells harness purinergenic signaling to tolerize human Th1 cells in vivo.
    Authors: Amarnath S, Foley J, Farthing D, Gress R, Laurence A, Eckhaus M, Metais J, Rose J, Hakim F, Felizardo T, Cheng A, Robey P, Stroncek D, Sabatino M, Battiwalla M, Ito S, Fowler D, Barrett A
    Stem Cells, 2015;33(4):1200-12.
    Species: Human
    Sample Types: Whole Cells
  10. CD39/adenosine pathway is involved in AIDS progression.
    Authors: Nikolova M, Carriere M, Jenabian MA, Limou S, Younas M, Kok A, Hue S, Seddiki N, Hulin A, Delaneau O, Schuitemaker H, Herbeck JT, Mullins JI, Muhtarova M, Bensussan A, Zagury JF, Lelievre JD, Levy Y
    PLoS Pathog., 2011;7(7):e1002110.
    Species: Human
    Sample Types: Cell Lysates
  11. Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response.
    Authors: Lei K, Townsend DM, Tew KD
    Oncogene, 2008;27(36):4877-87.
    Species: Human
    Sample Types: Cell Lysates
  12. NCAM induces CaMKIIalpha-mediated RPTPalpha phosphorylation to enhance its catalytic activity and neurite outgrowth.
    Authors: Bodrikov V, Sytnyk V, Leshchyns'ka I, den Hertog J, Schachner M
    J. Cell Biol., 2008;182(6):1185-200.
    Species: N/A
    Sample Types: Beads
  13. Moderate consumption of red wine (cabernet sauvignon) improves ischemia-induced neovascularization in ApoE-deficient mice: effect on endothelial progenitor cells and nitric oxide.
    Authors: Lefevre J, Michaud SE, Haddad P, Dussault S, Menard C, Groleau J, Turgeon J, Rivard A
    FASEB J., 2007;21(14):3845-52.
    Species: Human
    Sample Types: Cell Lysates
  14. Akt-mediated activation of HIF-1 in pulmonary vascular endothelial cells by S-nitrosoglutathione.
    Authors: Carver DJ, Gaston B, Deronde K, Palmer LA
    Am. J. Respir. Cell Mol. Biol., 2007;37(3):255-63.
    Species: Bovine
    Sample Types: Cell Lysates
  15. Enzymatic basis for N-glycan sialylation: structure of rat alpha2,6-sialyltransferase (ST6GAL1) reveals conserved and unique features for glycan sialylation.
    Authors: Meng L, Forouhar F, Thieker D, Gao Z, Ramiah A, Moniz H, Xiang Y, Seetharaman J, Milaninia S, Su M, Bridger R, Veillon L, Azadi P, Kornhaber G, Wells L, Montelione G, Woods R, Tong L, Moremen K
    J Biol Chem, 0;288(48):34680-98.
    Species: Rat
    Sample Types: Protein


  1. Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?

    • The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.

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Malachite Green Phosphate Detection Kit
By Anonymous on 09/07/2017
Application: Sample Tested: Recombinant protein