Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261), CF

A New rhCD40 Ligand is Available! ~1000 fold better activity; HEK293 expressed; HA tag!
New Product 6420-CL/CF.
(3 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. Spriggs, M.K. et al. (1992) J. Exp. Med. 176:1543. The ED50 for this effect is 1‑3 μg/mL in the presence of 20 ng/mL of recombinant human IL‑4.
  • Source
    E. coli-derived Glu108-Leu261, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    16.9 kDa (monomer)
  • SDS-PAGE
    18 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6245-CL/CF
 
6245-CL
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) (Catalog #
6245-CL/CF) stimulates cell proliferation using B cell-enriched peripheral blood mononuclear cells (PBMC) in the presence of IL-4. The ED50 for this effect is 1-3 μg/mL in the presence of 20 ng/mL of Recombinant Human IL‑4 (Catalog #
204-IL).
1 μg/lane of Recombinant Human CD40 Ligand/TNFSF5 (aa 108-261) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Background: CD40 Ligand/TNFSF5

Human CD40 LigandCD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily (1-3). Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region (4, 5). The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms (6-9). The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable (8). CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells (10). The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells (11).  CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (12, 13). CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS (14, 15). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (16, 17).

 

  • References:
    1. Zhang, G. (2004) Curr. Opin. Struct. Biol. 14:154.
    2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
    3. Quezada, S.A. et al. (2004) Annu. Rev. Immunol. 22:307.
    4. Graf, D. et al. (1992) Eur. J. Immunol. 22:3191.
    5. Hollenbaugh, D. et al. (1992) EMBO J. 11:4313.
    6. Khandekar, S.S. et al. (2001) Protein Expr. Purif. 23:301.
    7. Pietravalle, F. et al. (1996) J. Biol. Chem. 271:5965.
    8. Garber, E. et al. (1999) J. Biol. Chem. 274:33545.
    9. Vakkalanka, R.K. et al. (1999) Arthritis Rheum. 42:871.
    10. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
    11. Eissner, G. et al. (2004) Cytokine Growth Factor. Rev. 15:353.
    12. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
    13. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
    14. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
    15. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
    16. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
    17. Hassan, G.S. et al. (2012) Immunobiology 217:521.
  • Entrez Gene IDs:
    959 (Human); 21947 (Mouse); 84349 (Rat)
  • Alternate Names:
    CD154 antigen; CD154; CD40 antigen ligand; CD40 Ligand; CD40-L; CD40LG; CD40LIGM; gp39; hCD40L; HIGM1; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TNFSF5IMD3; TRAP; TRAPtumor necrosis factor (ligand) superfamily, member 5 (hyper-IgM syndrome); tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Modular expression analysis reveals functional conservation between human Langerhans cells and mouse cross-priming dendritic cells.
    Authors: Artyomov M, Munk A, Gorvel L, Korenfeld D, Cella M, Tung T, Klechevsky E
    J Exp Med, 2015;212(5):743-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Characterization of platelet-monocyte complexes in HIV-1-infected individuals: possible role in HIV-associated neuroinflammation.
    Authors: Singh, Meera V, Davidson, Donna C, Jackson, Joseph W, Singh, Vir B, Silva, Jharon, Ramirez, Servio H, Maggirwar, Sanjay B
    J Immunol, 2014;192(10):4674-84.
    Species: Human
    Sample Type: Whole Blood
    Application: Bioassay
  3. IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation.
    Authors: Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P
    PLoS Biol, 2012;10(9):e1001395.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

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