Recombinant Human CD58/LFA-3 Fc Chimera Protein, CF Summary
Accession # P19256
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human CD58 Fc Chimera (Catalog # 10068-CD) induces PHA-stimulated human T lymphoblasts proliferation. The ED50 for this effect is 0.3-1.8 μg/mL.
2 μg/lane of Recombinant Human CD58/LFA‑3 Fc Chimera (Catalog# 10068-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-82 kDa and 140 -160 kDa, respectively.
T cells require a signal induced by the engagement of the T cell receptor and a "co-stimulatory" signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58 and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4). Blockade of CD58-CD2 interaction on NK cells resulted in diminished production of IFN-gamma and TNF-alpha in response to Human cytomegalovirus (HCMV)-infected cells (7). Expression of CD58 was significantly increased in anisomycin-treated hepatocellular carcinoma (HCC), and may be critical player in NK-mediated immunotherapeutic effects (8).
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