Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10301-SR-050
Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF SDS-PAGE
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Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human S100A8 (Catalog # 9876-S8) is immobilized at 10 µg/mL (100 µL/well), it binds to Recombinant Human CD68/SR‑D1 Fc Chimera with an ED50 of 5-30 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CD68/SR-D1 protein
Human CD68/SR-D1
(Asn22-Ile320)
Accession # P34810
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asn22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
58 kDa
SDS-PAGE
116-130 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10301-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD68/SR-D1 Fc Chimera (Catalog # 10301-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 116-130 kDa and 230-260 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD68/SR-D1

CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), with a 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aa transmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, human CD68 shares a 65% and 67% amino acid sequence identity with that of mouse and rat CD68, respectively. CD68 is expressed on monocytes and tissue macrophages and serves as a scavenger receptor for oxidized LDL (2). In contrast to other members of the LAMP-family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appeared to have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is also a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68 influences the functions of cells through NF-kB/focal adhesion kinase pathway (7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity (8).

References
  1. Holness, C.L., Simmons, D.L. (1993) Blood 81:1607.
  2. Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
  3. Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453
  4. Chistiakov, D. et al. (2016) Lab Invest. 97:13.
  5. Bresnihan, B. et al. (2009) J Rheumatol. 36:1800.
  6. Touati, M. et al. (2015) 56:332.
  7. Wang, H. et al. (2014) Cancer Lett. 345:91.
  8. Okada, K. et al. (2016) J. of Leukoc. Bio. 100:1093.
Entrez Gene IDs
968 (Human); 12514 (Mouse); 287435 (Rat)
Alternate Names
CD68 antigenmacrophage antigen CD68; CD68 molecule; CD68; DKFZp686M18236; gp110; Macrosialin; SCARD1; scavenger receptor class D, member 1; SRD1; SR-D1

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