Recombinant Human CD68/SR-D1 Fc Chimera Protein, CF Summary
|Human CD68/SR-D1 |
Accession # P34810
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human CD68/SR-D1 Fc Chimera (Catalog # 10301-SR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 116-130 kDa and 230-260 kDa, respectively.
CD68, also called Scavenger Receptor D1 (SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoprotein that belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), with a 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aa transmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, human CD68 shares a 65% and 67% amino acid sequence identity with that of mouse and rat CD68, respectively. CD68 is expressed on monocytes and tissue macrophages and serves as a scavenger receptor for oxidized LDL (2). In contrast to other members of the LAMP-family of glycoproteins which are ubiquitously expressed in all cell types, CD68 appeared to have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is also a biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68 influences the functions of cells through NF-kB/focal adhesion kinase pathway (7). In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 to enhance the cell immunity (8).
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- Ramprasad, M.P. et al. (1996) Proc. Natl. Acad. Sci. U. S. A. 93:14838.
- Gottfried, E. et al. (2008) Scand. J. Immunol. 67:453
- Chistiakov, D. et al. (2016) Lab Invest. 97:13.
- Bresnihan, B. et al. (2009) J Rheumatol. 36:1800.
- Touati, M. et al. (2015) 56:332.
- Wang, H. et al. (2014) Cancer Lett. 345:91.
- Okada, K. et al. (2016) J. of Leukoc. Bio. 100:1093.
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