Recombinant Human CD69 Protein, CF Summary
Gly64-Lys199 with an N-terminal 9-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human CD69 is coated at 5 µg/mL (100 µL/well), the concentration of Recombinant Human Galectin-1 Recombinant Human Galectin-1 (Catalog # 1152-GA) that produces a 50% optimal binding response is 0.4-2 µg/mL.
1 μg/lane of Recombinant Human CD69 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 22.0, 27.9 kDa and NR bands at 39.3, 42.1, 47.2 kDa.
CD69, also known as CLEC2C, is a type 2 transmembrane glycoprotein in the C-type lectin family. It plays roles in immune cell trafficking, inflammation, T cell memory, and humoral immune responses (1). Mature human CD69 consists of a 40 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 138 aa extracellular domain with one C-type lectin domain (CTL) (2-4). Within the ECD, human CD69 shares 57% aa sequence identity with mouse and rat CD69. CD69 is expressed on the cell surface as an approximately 60 kDa disulfide-linked homodimer (3-5). It is found on CD4+ T cells, CD8+ T cells, NK cells, NKT cells, gamma delta cells dendritic cells (DC) and is up-regulated on activated T cells and DC (6-8). Ligation of CD69 on DC induces IL-2 production, leading to T cell proliferation (6). CD69 is important for the homing of CD4+ T cells and plasmablasts to the bone marrow but inhibits the migration of dermal DC to draining lymph nodes (7, 9). It supports the expression of multiple chemokines and chemokine receptors but suppresses the expression of others (10, 11). It associates with and negatively regulates S1P1 expression on DC and CD4+ T cells, resulting in a decreased chemotactic response to S1P (7, 12, 13). S1P1 similarly inhibits the cell surface expression of CD69 (12). The direct interaction of CD69 with Galectin-1 contributes to the ability of CD69 to limit Th17 mediated inflamamtion while supporting the differentiation of regulatory T cells (8, 10, 13-16). In various disease models, CD69 has been shown to have both enhancing and inhibitory effects on Th17 and Th2 cell mediated inflammation (8, 10, 11, 13, 16, 17).
- Gonzalez-Amaro, R. et al. (2013) Trends Mol. Med. 19:625.
- Ziegler, S.F. et al. (1993) Eur. J. Immunol. 23:1643.
- Hamann, J. et al. (1993) J. Immunol. 150:4920.
- Lopez-Cabrera, M. et al. (1993) J. Exp. Med. 178:537.
- Bieber, T. et al. (1992) J. Invest. Dermatol. 98:771.
- Alari-Pahissa, E. et al. (2012) J. Leukoc. Biol. 92:145.
- Lamana, A. et al. (2011) J. Invest. Dermatol. 131:1503.
- Radulovic, K. et al. (2012) J. Immunol. 188:2001.
- Shinoda, K. et al. (2012) Proc. Natl. Acad. Sci. USA 109:7409.
- Radulovic, K. et al. (2013) PLoS ONE 8:e65413.
- Hasegawa, A. et al. (2013) PLoS ONE 8:e65494.
- Shiow, L.R. et al. (2006) Nature 440:540.
- Martin, P. et al. (2010) J. Allergy Clin. Immunol. 126:355.
- Martin, P. et al. (2010) Mol. Cell. Biol. 30:4877.
- de la Fuente, H. et al. (2014) Mol. Cell. Biol. 34:2479.
- Cruz-Adalia, A. et al. (2010) Circulation 122:1396.
- Miki-Hosokawa, T. et al. (2009) J. Immunol. 183:8203.
Citation for Recombinant Human CD69 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Discovery, optimization and biodistribution of an Affibody molecule for imaging of CD69
Authors: J Persson, E Puuvuori, B Zhang, I Velikyan, O Åberg, M Müller, PÅ Nygren, S Ståhl, O Korsgren, O Eriksson, J Löfblom
Scientific Reports, 2021;11(1):19151.
Sample Types: Recombinant Proteins
Applications: Surface Plasmon Resonance
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