Recombinant Human CD79B Protein, CF

R&D Systems | Catalog # 9687-CD

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human CD79B Protein (9687-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P40259-1
View all CD79B Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD79B protein
Ala29-Asp159, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala29

Predicted Molecular Mass

16 kDa

SDS-PAGE

21 - 44 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human CD79B Protein, CF

Recombinant Human CD79B Protein SDS-PAGE

Recombinant Human CD79B Protein SDS-PAGE

2 μg/lane of Recombinant Human CD79B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 21 - 44 kDa.

Formulation, Preparation, and Storage

9687-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD79B

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79A. This complex interacts non-covalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79AB initiates signaling (1). Mature human CD79B is a 201 amino acid (aa) type I transmembrane glycoprotein (aa 29‑229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 185-213) (2). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation (3). Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.

References

  1. Tseng, J. et al. (1997). Blood 89:1513.
  2. Radaev, S, et al. (2010) Structure 18:934.
  3. Hashimoto, S. et al. (1995) Mol. Immunol. 32:651.

Alternate Names

B29, CD79B, IGB

Entrez Gene IDs

974 (Human); 15985 (Mouse); 171055 (Rat); 102145117 (Cynomolgus Monkey)

Gene Symbol

CD79B

UniProt

P40259-1

Additional CD79B Products

Product Documents for Recombinant Human CD79B Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD79B Protein, CF

For research use only

Related Research Areas

B Cell Activation

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