Recombinant Human CEACAM-1/CD66a Protein, CF

Catalog # Availability Size / Price Qty
2244-CM-050
Product Details
Citations (1)
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Recombinant Human CEACAM-1/CD66a Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544. rhCEACAM-1 at concentrations higher than 1 µg/mL reduces anti-CD3 (immobilized at 10 µg/mL) induced IL-2 production by 60-70%.
The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is 0.05-0.2 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human CEACAM-1/CD66a protein
Gln35-Gly428, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Gln35
Predicted Molecular Mass
44.6 kDa
SDS-PAGE
100-130 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2244-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CEACAM-1/CD66a

Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1 - 3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3 & 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1 - 3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6, 7, 8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35 - 142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145 - 317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V-type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear.
Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V-type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its LeX moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

References
  1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
  2. Thompson, J. et al. (1992) Genomics 12:761.
  3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
  4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
  5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
  6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
  7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
  8. Watt, S.M. et al. (1994) Blood 84:200.
  9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
  10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
  11. Bogoevska, V. et al. (2005) Glycobiology 16:197.
Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 1
Entrez Gene IDs
634 (Human); 26365 (Mouse)
Alternate Names
antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; CEACAM1; CEACAM-1; Hv-1; Hv-2; MHVR

Citation for Recombinant Human CEACAM-1/CD66a Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cytokine-induced CEACAM1 expression on keratinocytes is characteristic for psoriatic skin and contributes to a prolonged lifespan of neutrophils.
    Authors: Rahmoun M, Moles JP, Pedretti N, Mathieu M, Fremaux I, Raison-Peyron N, Lecron JC, Yssel H, Pene J
    J. Invest. Dermatol., 2009;129(3):671-81.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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