Recombinant Human CNTFR/CNTF Complex Protein, CF

Up to 100-fold greater activity than CNTF alone
Catalog # Availability Size / Price Qty
10283-CC-020
10283-CC-050
Recombinant Human CNTFR/CNTF Complex Protein, CF Bioactivity
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Recombinant Human CNTFR/CNTF Complex Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.25-1.5 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human CNTFR/CNTF Complex protein
Human CNTFR
(Gln23-Ser342)
Accession # P26992
GGGSGGGSGGGS  Human CNTF
(Met1-Met200)
Accession # P26441
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Gln23 inferred from deblocking revealing Arg24
Predicted Molecular Mass
59 kDa
SDS-PAGE
74-85 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10283-CC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human CNTFR/CNTF Complex (Catalog # 10283-CC) stimulates proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.25-1.5 ng/mL. The Recombinant Human CNTFR/CNTF Complex has up to 100-fold greater activity than Recombinant CNTF alone (Catalog # 257-NT).

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human CNTFR/CNTF Complex was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CNTFR/CNTF Complex

Human CNTF (ciliary neurotrophic factor) encodes a 200 amino acid polypeptide that lacks a signal sequence. It shares 83% and 84% sequence identity with the mouse and rat homologs, respectively. CNTF is known to be expressed by Schwann cells, astrocytes and T cells (1). CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes (2, 3). Engagement of CNTF with CNTFR alpha, LIFR, and gp130 on CD4 T cells has been shown to increase the number of IFN-gamma producing differentiated cells. IFN-gamma being a mediator of IFN-beta suggests CNTF's role in therapeutic effects of IFN-beta in multiple sclerosis (1, 4). CNTFR alpha contains a 22 amino acid signal peptide, 320 amino acids mature domain and a 30 amino acids propeptide. The mature human CNTFR alpha contains one Ig-like C2-type domain and two Fibronectin type III domains. CNTFR alpha differs from other cytokine receptors in that it lacks transmembrane and cytoplasmic domains and is anchored to cell membranes by a glycosylphosphatidylinositol (GPI) linkage (5). Mature human CNTFR alpha shares 98% and 96% sequence identity with its mouse and rat homologs in the mature domain, respectively. Similar to other GPI-linked proteins, soluble CNTFR alpha (CNTF sR alpha ) can be released from the cell surface by phosphatidylinositol-specific phospholipase C. CNTF sR alpha can be released from skeletal muscle in response to peripheral nerve injury and high concentrations of CNTF sR alpha have also been detected in human cerebrospinal fluid (4). CNTF sR alpha binds CNTF in solution and the complex can act on cells that express only LIF R beta and gp130 but not CNTFR alpha (4, 6).

References
  1. Tormo, A.J. et al. (2012) Cytokine 60:65.
  2. Sleeman, M.W. et al. (2000). Pharma Acta Helv. 74:265.
  3. Bravo J. et al. (2000) EMBO J. 19:2399
  4. Pasquin, S. et al. (2015) Cytokine Growth Factor 26:507.
  5. Davis, S. et al. (1991). Science. 253:59.
  6. Pasquin, S. et al. (2016) Cytokine 82:122.
Long Name
Ciliary Neurotrophic Factor Receptor alpha/Ciliary Neurotrophic Factor Complex
Alternate Names
CNTFR/CNTF Complex

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