Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF

Catalog # Availability Size / Price Qty
9398-TN-025/CF
Recombinant Human CTRP3/C1qTNF3/CORS26 Protein Binding Activity
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Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinan Human CTRP3/C1qTNF3/CORS26 is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human CD36/SR-B3 Fc Chimera (Catalog # 1955-CD) that produces 50% of the optimal binding response is 1.5-7.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human CTRP3/C1qTNF3/CORS26 protein
Gln23-Lys246
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln23 inferred from enzymetic pyroglutamate treatment revealing Asp24
Structure / Form
Disulfide-linked homo-oligomer
Predicted Molecular Mass
24 kDa
SDS-PAGE
25-37 kDa, reducing conditions

Product Datasheets

9398-TN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9398-TN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Human CTRP3/C1qTNF3/CORS26 Protein Binding Activity View Larger

When Recombinant Human CTRP3/C1qTNF3/CORS26 (Catalog # 9398-TN) is immobilized at 4 µg/mL, Recombinant Human CD36/SR-B3 Fc Chimera (Catalog # 1955-CD) binds with an ED50 of 1.5-7.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CTRP3/C1qTNF3/CORS26

CORS-26 (collagenous repeat-containing sequence of 26 kDa protein) also known as C1qTNF3 (complement C1q TNF-related protein 3/CTRP3), cartonectin, cartducin, is a 30-32 kDa, secreted member of the C1q and TNF-related (CTRP) superfamily of molecules (1). The mature protein is 224 aa in length. It contains an N-terminal collagen-like domain followed by a C-terminal globular region. Human CORS26 shares 99% aa sequence identity with the mouse CORS26 (2). Like other CTRP members, CORS26 has a trimeric structure and can assemble into hexameric or higher order molecular forms (3). It is expressed by a variety of cells, including adipocytes, cartilage, fibroblasts, monocytes and proliferating chondrocytes (4). The inflammatory effects of LPS, TLR-4 and fatty acids have been shown to be inhibited by CORS26 in adipocytes and monocytes (5). In mouse models, CORS26 has been shown to lower glucose levels and decrease gluconeogenic gene expression (6). Inhibition of 3T3-L1 pre-adipocyte differentiation to adipocytes is associated with CORS26 treatment, demonstrating potential anti-obesity effects (7). Treatment with CORS26 results in the proliferation of skeletal muscle C2C12 cells and inhibition of C2C12 myotube differentiation, mediated by the ERK pathway (8). Due to the variety of functions in metabolism and inflammation, CORS26 is a potential new target of type 2 diabetes treatment.

References
  1. Wong, G.W. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:10302.
  2. Schaffler A. et al. (2003). Biochim Biophys Acta.1630:123.
  3. Suzuki S. et al. (2007) FEBS Lett. 581: 809.
  4. Weigert J. et al. (2005) FEBS Lett. 579: 5565.
  5. Kopp, A. et al. (2010) Endocrinology. 151:5267.
  6. Peterson J. M. et al. (2010) J Biol Chem. 285:39691.
  7. Nishimoto, H. et al. (2017) Cell Bio Int. 41:197.
  8. Otani M. et al. (2015) Mol Cell Biochem. 409:271.
Long Name
C1q and Tumor Necrosis Factor-related Protein 3
Entrez Gene IDs
114899 (Human); 81799 (Mouse)
Alternate Names
C1ATNF3; C1q and tumor necrosis factor related protein 3; C1qTNF3; Cartducin; cartonectin; collagenous repeat-containing sequence of 26-kDa; complement C1q tumor necrosis factor-related protein 3; complement-c1q tumor necrosis factor-related protein 3; Corcs; Cors; CORS26; Cors-26; CTRP3; CTRP32310005P21Rik; FLJ37576; Secretory protein CORS26

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