CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF‑ alpha, IL-12 and viruses. Additional cell types that have been shown to express CXCL10 include activated T‑lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T‑lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.
Recombinant Human CXCL10/IP-10 Protein
R&D Systems | Catalog # 266-IP
Key Product Details
- R&D Systems E. coli-derived Recombinant Human CXCL10/IP-10 Protein (266-IP)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Val22-Pro98, with an N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 0.03‑0.18 µg/mL.
Reviewed Applications
Read 3 reviews rated 5 using 266-IP in the following applications:
Scientific Data Images for Recombinant Human CXCL10/IP-10 Protein
Recombinant Human CXCL10/IP-10 Protein Bioactivity
Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3. The ED50 for this effect is 0.03-0.18 μg/mL.
Recombinant Human CXCL10/IP-10 Protein SDS-PAGE
1 µg/lane of Recombinant Human CXCL10/IP-10/CRG-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 266-IP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 266-IP/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: CXCL10/IP-10/CRG-2
References
- Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
- Wang, X. et al.(1996) J. Biol. Chem. 271:24286.
Alternate Names
Gene Symbol
UniProt
Additional CXCL10/IP-10/CRG-2 Products
Product Documents for Recombinant Human CXCL10/IP-10 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human CXCL10/IP-10 Protein
For research use only
Citations for Recombinant Human CXCL10/IP-10 Protein
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Customer Reviews for Recombinant Human CXCL10/IP-10 Protein (3)
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Customer Images
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/11/2025
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Application: Immunoassay StandardVerified Customer | Posted 08/17/2018
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Application: Immunoassay StandardVerified Customer | Posted 01/05/2018This recombinant protein was used as a standard in an ELISA for IP-10.
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