Recombinant Human CXCL10/IP-10 Protein

Formulations:
Catalog # Availability Size / Price Qty
266-IP-010
266-IP-050
Recombinant Human CXCL10/IP-10 Protein Data
2 Images
Product Details
Citations (44)
FAQs
Reviews (2)

Recombinant Human CXCL10/IP-10 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED50 for this effect is 0.03‑0.18 µg/mL.
Source
E. coli-derived human CXCL10/IP-10/CRG-2 protein
Val22-Pro98, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
8.7 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

266-IP

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

266-IP/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Human CXCL10/IP-10/CRG-2 (Catalog # 266-IP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3. The ED50for this effect is 0.03-0.18 μg/mL.

SDS-PAGE View Larger

1 µg/lane of Recombinant Human CXCL10/IP-10/CRG-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 9 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: CXCL10/IP-10/CRG-2

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF‑ alpha, IL-12 and viruses. Additional cell types that have been shown to express CXCL10 include activated T‑lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, Crg-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T‑lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T‑lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Wang, X. et al.(1996) J. Biol. Chem. 271:24286.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

Citations for Recombinant Human CXCL10/IP-10 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Mitochondrial mass governs the extent of human T cell senescence
    Authors: LA Callender, EC Carroll, EA Bober, AN Akbar, E Solito, SM Henson
    Aging Cell, 2019;0(0):e13067.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow
    Authors: M Goedhart, S Gessel, R van der Vo, E Slot, B Lucas, E Gielen, M Hoogenboez, T Rademakers, S Geerman, JD van Buul, S Huveneers, H Dolstra, G Anderson, C Voermans, MA Nolte
    Eur. J. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Effect of a Combination of Prednisone or Mycophenolate Mofetil and Mesenchymal Stem Cells on Lupus Symptoms in MRL.Faslpr Mice
    Authors: HK Lee, KH Kim, HS Kim, JS Kim, JH Lee, A Ji, KS Kim, TY Lee, IY Chang, SC Bae, JT Hong, Y Kim, SB Han
    Stem Cells Int, 2018;2018(0):4273107.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling
    Authors: M Liu, W Solomon, JC Cespedes, NO Wilson, B Ford, JK Stiles
    J Neuroinflammation, 2018;15(1):104.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Upregulation of chemokine CXCL10 enhances chronic pulmonary inflammation in tree shrew collagen-induced arthritis
    Authors: B Gao, J Lin, Z Jiang, Z Yang, H Yu, L Ding, M Yu, Q Cui, N Dunavin, M Zhang, M Li
    Sci Rep, 2018;8(1):9993.
    Species: Tree Shrew
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Engineering the haemogenic niche mitigates endogenous inhibitory signals and controls pluripotent stem cell-derived blood emergence
    Authors: N Rahman, PM Brauer, L Ho, T Usenko, M Tewary, JC Zúñiga-Pfl, PW Zandstra
    Nat Commun, 2017;8(0):15380.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Islet inflammation and ductal proliferation may be linked to increased pancreatitis risk in type 2 diabetes
    Authors: B Schludi, ASM Moin, C Montemurro, T Gurlo, AV Matveyenko, D Kirakossia, DW Dawson, SM Dry, PC Butler, AE Butler
    JCI Insight, 2017;2(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells
    Authors: Z Jiang, Y Li, X Ji, Y Tang, H Yu, L Ding, M Yu, Q Cui, M Zhang, Y Ma, M Li
    Sci Rep, 2017;7(1):14510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
    Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
    Oncol Lett, 2017;13(6):4224-4230.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization
    Authors: Valentina Cecchinato
    J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Serum level of CXCL10 is associated with inflammatory prognostic biomarkers in patients with diffuse large B-cell lymphoma
    Authors: Jung Yong Hong
    Hematol Oncol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
    Mucosal Immunol, 2016;9(6):1584-1595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. TNF-alpha induces CXCL1 chemokine expression and release in human vascular endothelial cells in vitro via two distinct signaling pathways.
    Authors: Lo H, Lai T, Li C, Wu W
    Acta Pharmacol Sin, 2014;35(3):339-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  15. Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
    Authors: Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J
    J Allergy Clin Immunol, 2014;133(4):1227-30.
    Species: Human
    Sample Types:
  16. Profibrotic activities for matrix metalloproteinase-8 during bleomycin-mediated lung injury.
    Authors: Craig V, Quintero P, Fyfe S, Patel A, Knolle M, Kobzik L, Owen C
    J Immunol, 2013;190(8):4283-96.
    Species: Mouse
    Sample Types: Protein
    Applications: Enzyme Assay
  17. Human trophectoderm apposition is regulated by interferon gamma-induced protein 10 (IP-10) during early implantation.
    Authors: Sela H, Goldman-Wohl D, Haimov-Kochman R, Greenfield C, Natanson-Yaron S, Hamani Y, Revel A, Lavy Y, Singer O, Yachimovich-Cohen N, Turetsky T, Mandelboim O, Reubinoff B, Yagel S
    Placenta, 2013;34(3):222-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Adenovirus-engineered human dendritic cells induce natural killer cell chemotaxis via CXCL8/IL-8 and CXCL10/IP-10.
    Authors: Vujanovic, Lazar, Ballard, Wenners, Thorne, Stephen, Vujanovic, Nikola L, Butterfield, Lisa H
    Oncoimmunology, 2012;1(4):448-457.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Chemokine receptor CXCR3 promotes growth of glioma.
    Authors: Liu C, Luo D, Reynolds BA
    Carcinogenesis, 2011;32(2):129-37.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Type I interferon signaling in hematopoietic cells is required for survival in mouse polymicrobial sepsis by regulating CXCL10.
    Authors: Kelly-Scumpia KM, Scumpia PO, Delano MJ, Weinstein JS, Cuenca AG, Wynn JL, Moldawer LL
    J. Exp. Med., 2010;207(2):319-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema.
    Authors: Shan M, Cheng HF, Song LZ
    Sci Transl Med, 2009;1(4):4ra10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. CXCR3 activation by lentivirus infection suppresses neuronal autophagy: neuroprotective effects of antiretroviral therapy.
    Authors: Zhu Y, Vergote D, Pardo C, Noorbakhsh F, McArthur JC, Hollenberg MD, Overall CM, Power C
    FASEB J., 2009;23(9):2928-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Potential role of chemerin in recruitment of plasmacytoid dendritic cells to diseased skin.
    Authors: Skrzeczynska-Moncznik J, Wawro K, Stefanska A, Oleszycka E, Kulig P, Zabel BA, Sulkowski M, Kapinska-Mrowiecka M, Czubak-Macugowska M, Butcher EC, Cichy J
    Biochem. Biophys. Res. Commun., 2009;380(2):323-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  28. Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.
    Authors: Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
    J. Immunol., 2007;179(7):4840-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  29. HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
    Authors: Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
    AIDS, 2007;21(10):1263-72.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  30. Expression and role of CCR6/CCL20 chemokine axis in pulmonary sarcoidosis.
    Authors: Facco M, Baesso I, Miorin M, Bortoli M, Cabrelle A, Boscaro E, Gurrieri C, Trentin L, Zambello R, Calabrese F, Cassatella MA, Semenzato G, Agostini C
    J. Leukoc. Biol., 2007;82(4):946-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  33. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  34. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  35. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
  36. Ras-induced modulation of CXCL10 and its receptor splice variant CXCR3-B in MDA-MB-435 and MCF-7 cells: relevance for the development of human breast cancer.
    Authors: Datta D, Flaxenburg JA, Laxmanan S, Geehan C, Grimm M, Waaga-Gasser AM, Briscoe DM, Pal S
    Cancer Res., 2006;66(19):9509-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
    Authors: Nguyen AN, Stebbins EG, Henson M, O&amp;amp;apos;Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
    Exp. Cell Res., 2006;312(10):1909-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  39. Ultraviolet radiation-induced injury, chemokines, and leukocyte recruitment: An amplification cycle triggering cutaneous lupus erythematosus.
    Authors: Meller S, Winterberg F, Gilliet M, Muller A, Lauceviciute I, Rieker J, Neumann NJ, Kubitza R, Gombert M, Bunemann E, Wiesner U, Franken-Kunkel P, Kanzler H, Dieu-Nosjean MC, Amara A, Ruzicka T, Lehmann P, Zlotnik A, Homey B
    Arthritis Rheum., 2005;52(5):1504-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Involvement of the CXCL12/CXCR4 pathway in the advanced liver disease that is associated with hepatitis C virus or hepatitis B virus.
    Authors: Wald O, Pappo O, Safadi R, Dagan-Berger M, Beider K, Wald H, Franitza S, Weiss I, Avniel S, Boaz P, Hanna J, Zamir G, Eid A, Mandelboim O, Spengler U, Galun E, Peled A
    Eur. J. Immunol., 2004;34(4):1164-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
    Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
    Blood, 2004;104(2):314-20.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  42. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  43. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  44. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001;31(4):1037-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human CXCL10/IP-10 Protein

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Recombinant Human CXCL10/IP-10 Protein
By Anonymous on 08/17/2018
Application: Immunoassay Standard

Recombinant Human CXCL10/IP-10 Protein
By Anonymous on 01/05/2018
Application: Immunoassay Standard

This recombinant protein was used as a standard in an ELISA for IP-10.