Recombinant Human CXCL11/I-TAC Protein

R&D Systems | Catalog # 672-IT

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems E. coli-derived Recombinant Human CXCL11/I-TAC Protein (672-IT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

O14625

Applications

Bioactivity
View all CXCL11/I-TAC Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CXCL11/I-TAC protein
Phe22-Phe94

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe22

Predicted Molecular Mass

8.3 kDa

Activity

Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3.
The ED50 for this effect is 1‑5 ng/mL.

Reviewed Applications

Read 2 reviews rated 4.5 using 672-IT in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 672-IT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 672-IT/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CXCL11/I-TAC

CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes. The gene encoding CXCL11 has been mapped to chromosome 4.

References

  1. Cole, K. et al. (1998) J. Exp. Med. 187:2009.
  2. Sandhya Rani, M. et al. (1996) J. Biol. Chem. 271:22878.
  3. Lou, Y. et al. (1998) J. Neurovirol. 4:575.

Alternate Names

beta-R1, H174, I-TAC, ITAC, SCYB9B

Entrez Gene IDs

6373 (Human); 56066 (Mouse)

Gene Symbol

CXCL11

UniProt

O14625

Additional CXCL11/I-TAC Products

Product Documents for Recombinant Human CXCL11/I-TAC Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 672-IT (with carrier)
Download SDS 672-IT/CF (carrier free)

Product Specific Notices for Recombinant Human CXCL11/I-TAC Protein

For research use only

Citations for Recombinant Human CXCL11/I-TAC Protein

Customer Reviews for Recombinant Human CXCL11/I-TAC Protein (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Recombinant Human CXCL11/I-TAC Protein?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Recombinant Human CXCL11/I-TAC Protein
    Name: Wuyi Wang
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/28/2020
  • Recombinant Human CXCL11/I-TAC Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/22/2019

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes