Recombinant Human DCC Protein, CF

Catalog # Availability Size / Price Qty
8637-DC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human DCC Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human DCC was immobilized at 1 μg/mL (100 μL/well), it binds Recombinant Mouse Netrin-1 (Catalog # 1109-N1) . The concentration of Recombinant Mouse Netrin-1 that produces 50% of the optimal binding response is approximately 5-30 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human DCC protein
Phe32-Asn1097, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Phe32
Predicted Molecular Mass
119 kDa
SDS-PAGE
123-138 kDa, reducing conditions

Product Datasheets

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8637-DC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8637-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DCC

Deleted in Colorectal Cancer (DCC) was originally identified as a putative tumor suppressor gene that is down-regulated in more than 70% of colorectal cancers (1). DCC is a type I transmembrane glycoprotein that belongs to the immunoglobulin (Ig) superfamily (2, 3). It contains an extracellular domain (ECD) composed of four Ig-like domains and six fibronectin type III repeats, a transmembrane region, and a cytoplasmic domain (1, 4-6). The ECD of human DCC shares 97% amino acid sequence identity with mouse and rat DCC. In adults, DCC is expressed most abundantly in brain tissue, but it is also expressed at low levels in multiple other tissues (4, 6). DCC is a receptor for Netrin-1 and is important for dopaminergic neuronal precursor migration, axon guidance, and axon arborization (2, 7-10). Binding of DCC by Draxin, at a site distinct from the Netrin-1 binding site, results in the inhibition of neurite outgrowth (11). Signaling downstream of DCC may also be required for the confinement of central axons to the central nervous system (12). DCC regulates apoptosis as a dependence receptor and induces apoptosis in the absence of Netrin-1 binding (13-15). DCC has been shown to inhibit metastasis, and loss of DCC’s apoptotic activity promotes tumorigenesis in mice, providing in vivo evidence that DCC is a tumor suppressor gene (16-18).

References
  1. Fearon, E.R. et al. (1990) Science 247:49.
  2. Keino-Masu, K. et al. (1996) Cell 87:175.
  3. Arakawa, H. (2004) Nat. Rev. Cancer 4:978.
  4. Hedrick, L. et al. (1994) Genes Dev. 8:1174.
  5. Vielmetter, J. et al. (1994) J. Cell Biol. 127:2009.
  6. Reale, M.A. et al. (1994) Cancer Res. 54:4493.
  7. Hong, K. et al. (1999) Cell 97:927.
  8. Forcet, C. et al. (2002) Nature 417:443.
  9. Xu, B. et al. (2010) Neuroscience 169:932.
  10. Zhang, C. et al. (2012) J. Neurosci. 32:12589.
  11. Ahmed, G. et al. (2011) J. Neurosci. 31:14018.
  12. Laumonnerie, C. et al. (2014) Development 141:594.
  13. Mehlen, P. et al. (1998) Nature 395:801.
  14. Chen, Y.Q. et al. (1999) Oncogene 18:2747.
  15. Furne, C. et al. (2008) Proc. Natl. Acad. Sci. USA 105:14465.
  16. Rodrigues, S. et al. (2007) Oncogene 26:5615.
  17. Krimpenfort, P. et al. (2012) Nature 482:538.
  18. Castets, M. et al. (2011) Nature 482:534.
Long Name
Deleted in Colorectal Cancer
Entrez Gene IDs
1630 (Human); 13176 (Mouse)
Alternate Names
Colorectal cancer suppressor; CRC18; CRCR1; DCC; deleted in colorectal cancer protein; deleted in colorectal carcinoma; IGDCC1colorectal tumor suppressor; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; netrin receptor DCC; Tumor suppressor protein DCC

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