Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF

HEK293 Expressed
Catalog # Availability Size / Price Qty
10364-DC-050
Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF Data
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Recombinant Human Dectin-1/CLEC7A Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) is immobilized at 0.25 µg/mL (100 µL/well), Biotinylated Laminarin (1, 3-beta-glucan) binds with an ED50 of 0.08-9.6 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Dectin-1/CLEC7A protein
MDHuman IgG1
(Pro100-Lys330)
IEGR Human Dectin-1/CLEC7A
(Thr66-Met247)
Accession # Q9BXN2.1
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis

Met Asp-Pro100

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
47 kDa
SDS-PAGE
54-64 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10364-DC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

When Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Laminarin (1, 3-beta-glucan) binds with an ED50of 0.08-9.6 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human Dectin-1/CLEC7A Fc Chimera (Catalog # 10364-DC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-64 kDa and 108-128 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (ECD) (3, 4). Alternate splicing generates one major isoform expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells that lacks the stalk region (3-6). The mature ECD of human Dectin-1 shares 59% and 57% amino acid (aa) sequence identity with mouse and rat Dectin-1, respectively. The CRD of Dectin-1 selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5-7). Yeast beta -glucan is accessible to Dectin-1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8-10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11-13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15, 16).

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
  4. Yokota, K. et al. (2001) Gene 272:51.
  5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
  6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

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