Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF

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Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL/CF) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human DR3/TNFRSF25 Fc Chimera binds with an ED50 of 0.185-2.40 μg/mL.
Mouse myeloma cell line, NS0-derived human DR3/TNFRSF25 protein
Human DR3
(Gln25-Phe201) & (Arg29-Phe201)
Accession # Q93038.2
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg29 & No results obtained: Gln25 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
46.6 kDa (monomer)
50-65 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: DR3/TNFRSF25

Death receptor 3 (DR3), also known as lymphocyte-associated receptor of death (LARD), WSL-1, APO3, TRAMP and TR3, is a glycoprotein belonging to the TNF receptor superfamily (TNFRSF) (1 - 5). DR3 was formerly designated TNFRSF12 when it was thought to be a receptor for TWEAK/TNFSF12 (6). However, work disavowed the DR3:TWEAK interaction and DR3 is now designated TNFRSF 25 (7). By alternative splicing, at least 11 distinct human DR3 transcripts encoding secreted or type I membrane proteins exist (7). The human DR3 isoform 1 cDNA encodes a 417 amino acid residue (aa) transmembrane precursor with a 24 aa signal peptide, a 175 aa extracellular domain containing four cysteine-rich repeats and two potential N-glycosylation sites, a 21 aa transmembrane region and a 195 aa cytoplasmic region with one death domain. DR3 is one of six within the TNF R superfamily that contains a death domain in its cytooplasmic region. It is most closely related to TNF R1 and FAS/CD95, sharing 29% and 23% aa sequence identity, respectively. DR3 is expressed primarily in tissues enriched in lymphocytes. Whereas naïve B and T cells express multiple truncated DR3 isoforms but not the transmembrane isoform 1, upon T cell activation, expression of the transmembrane DR3 isoform 1 predominates. TL1A/VEGI, a TNF superfamily ligand, has been shown to bind and activate DR3 (8). Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. In DR3-null mice, an impairment of negative selection and anti-CD3-mediated thymocyte apoptosis is observed.

  1. Chinnaiyan, A.M., et al. (1996) Science 274:990.
  2. Kitson, J., et al. (1996) Nature 384:372.
  3. Bodmer, J-L., et al. (1997) Immunity 6:79.
  4. Screaton, G.R. et al. (1997) Proc. Nat. Acad. Sci. USA 94:4615.
  5. Marsters, S.A. et al. (1996) Curr. Biol. 6:1669.
  6. Marsters, S.A. et al. (1998) Curr. Biol. 8:525.
  7. Kaptein, A. et al. (2000) FEBS Lett. 485:135.
  8. Migone, T-S. et al. (2002) Immunity 16:479.
Long Name
Death Receptor 3
Entrez Gene IDs
8718 (Human); 85030 (Mouse)
Alternate Names
APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1

Citations for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. A stable, engineered TL1A ligand co-stimulates T cells via specific binding to DR3
    Authors: A Zwolak, SR Chan, P Harvilla, S Mahady, AA Armstrong, L Luistro, N Tamot, D Yamada, M Derebe, S Pomerantz, M Chiu, R Ganesan, P Chowdhury
    Scientific Reports, 2022-11-29;12(1):20538.
    Species: Human
    Sample Types:
    Applications: ELISA Development
  2. TL1A-DR3 Plasma Levels Are Predictive of HIV-1 Disease Control, and DR3 Costimulation Boosts HIV-1-Specific T Cell Responses
    Authors: B Oriol-Tord, A Olvera, C Duran-Cast, A Llano, B Mothe, M Massanella, J Dalmau, C Ganoza, J Sanchez, ML Calle, B Clotet, J Martinez-P, E Negredo, J Blanco, D Hartigan-O, C Brander, M Ruiz-Riol
    J Immunol, 2020-11-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  4. Directed evolution of a soluble human DR3 receptor for the inhibition of TL1A induced cytokine secretion
    Authors: I Levin, M Zaretsky, A Aharoni
    PLoS ONE, 2017-03-09;12(3):e0173460.
    Species: Human
    Sample Types: N/A
    Applications: ELISA Developmet
  5. A TNFSF15 disease-risk polymorphism increases pattern-recognition receptor-induced signaling through caspase-8-induced IL-1.
    Authors: Hedl M, Abraham C
    Proc Natl Acad Sci U S A, 2014-09-02;111(37):13451-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Progranulin-derived Atsttrin directly binds to TNFRSF25 (DR3) and inhibits TNF-like ligand 1A (TL1A) activity.
    Authors: Liu, Cui, Li, Xing-Xia, Gao, Wei, Liu, Wen, Liu, De-Shan
    PLoS ONE, 2014-03-20;9(3):e92743.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  7. Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells.
    Authors: Krysov SV, Krysov</LastName><ForeNam SV</Initia, Rowley TF, Al-Shamkhani A
    Mol. Cancer Ther., 2007-02-01;6(2):703-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Reviews for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF

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Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
By Anonymous on 03/24/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
By Anonymous on 02/16/2018
Application: Analytical SEC
Reason for Rating: 82.6% dimer