Death receptor 3 (DR3), also known as lymphocyte-associated receptor of death (LARD), WSL-1, APO3, TRAMP and TR3, is a glycoprotein belonging to the TNF receptor superfamily (TNFRSF) (1 - 5). DR3 was formerly designated TNFRSF12 when it was thought to be a receptor for TWEAK/TNFSF12 (6). However, work disavowed the DR3:TWEAK interaction and DR3 is now designated TNFRSF 25 (7). By alternative splicing, at least 11 distinct human DR3 transcripts encoding secreted or type I membrane proteins exist (7). The human DR3 isoform 1 cDNA encodes a 417 amino acid residue (aa) transmembrane precursor with a 24 aa signal peptide, a 175 aa extracellular domain containing four cysteine-rich repeats and two potential N-glycosylation sites, a 21 aa transmembrane region and a 195 aa cytoplasmic region with one death domain. DR3 is one of six within the TNF R superfamily that contains a death domain in its cytooplasmic region. It is most closely related to TNF R1 and FAS/CD95, sharing 29% and 23% aa sequence identity, respectively. DR3 is expressed primarily in tissues enriched in lymphocytes. Whereas naïve B and T cells express multiple truncated DR3 isoforms but not the transmembrane isoform 1, upon T cell activation, expression of the transmembrane DR3 isoform 1 predominates. TL1A/VEGI, a TNF superfamily ligand, has been shown to bind and activate DR3 (8). Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. In DR3-null mice, an impairment of negative selection and anti-CD3-mediated thymocyte apoptosis is observed.
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Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
R&D Systems | Catalog # 943-D3
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Key Product Details
- R&D Systems NS0-derived Recombinant Human DR3/TNFRSF25 Fc Chimera Protein (943-D3)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Binding Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived human DR3/TNFRSF25 protein
| Human DR3 (Gln25-Phe201) & (Arg29-Phe201) Accession # Q93038.2 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg29 & No results obtained: Gln25 predicted
Predicted Molecular Mass
46.6 kDa (monomer)
SDS-PAGE
50-65 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL/CF) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human DR3/TNFRSF25 Fc Chimera binds with an ED50 of 0.185-2.40 μg/mL.
When Recombinant Human TL1A/TNFSF15 (Catalog # 1319-TL/CF) is immobilized at 2 μg/mL (100 μL/well), Recombinant Human DR3/TNFRSF25 Fc Chimera binds with an ED50 of 0.185-2.40 μg/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 943-D3 in the following applications:
Formulation, Preparation, and Storage
943-D3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: DR3/TNFRSF25
References
- Chinnaiyan, A.M., et al. (1996) Science 274:990.
- Kitson, J., et al. (1996) Nature 384:372.
- Bodmer, J-L., et al. (1997) Immunity 6:79.
- Screaton, G.R. et al. (1997) Proc. Nat. Acad. Sci. USA 94:4615.
- Marsters, S.A. et al. (1996) Curr. Biol. 6:1669.
- Marsters, S.A. et al. (1998) Curr. Biol. 8:525.
- Kaptein, A. et al. (2000) FEBS Lett. 485:135.
- Migone, T-S. et al. (2002) Immunity 16:479.
Long Name
Death Receptor 3
Alternate Names
Apo-3, LARD, TNFRSF25, TRAMP, WSL-1
Gene Symbol
TNFRSF25
UniProt
Additional DR3/TNFRSF25 Products
Product Documents for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF
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Customer Reviews for Recombinant Human DR3/TNFRSF25 Fc Chimera Protein, CF (2)
4.5 out of 5
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Associated Pathways
TNF Superfamily Pathway: Human Ligand-Receptor Interactions & their Associated Functions
