Recombinant Human TL1A/TNFSF15 Protein

Carrier Free

Catalog # Availability Size / Price Qty
1319-TL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
1319-TL-010

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Citations (13)
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Recombinant Human TL1A/TNFSF15 Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce apoptosis of TF‑1 human erythroleukemic cells. Migone, T. et al. (2002) Immunity 16:479. The ED50 for this effect is 4-20 ng/mL.
Source
E. coli-derived human TL1A/TNFSF15 protein
Leu72-Leu251, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21 kDa (monomer)

Product Datasheets

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1319-TL (with carrier)

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1319-TL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1319-TL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1319-TL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: TL1A/TNFSF15

TL1A is a type II transmembrane protein belonging to the TNF superfamily and has been designated TNF superfamily member 15 (TNFSF15). Human TL1A is a 251 aa protein consisting of a 35 aa cytoplasmic domain, a 24 aa transmembrane region and a 192 aa C-terminal extracellular domain. It is a longer variant of the previously cloned TL1 (also known as VEGI) that is possibly a cloning artifact. TL1A is predominantly expressed in endothelial cells and its expression is inducible by TNF-alpha and IL-1 alpha. TL1A binds with high affinity to death receptor 3 (DR3), which is now designated TNF receptor superfamily member 25 (TNFRSF25). DR3 was formerly designated TNFRSF12 when it was thought to be the receptor for TWEAK/TNFSF12. DR3 is expressed primarily on activated T cells. Depending on the cell context, ligation of DR3 by TL1A can trigger one of two signaling pathways, activation of the transcription factor NF-kappa-B or activation of caspases and apoptosis. On primary T cells, TL1A induces NF-kappa-B activation and a costimulatory signal to increase IL-2 responsiveness and the secretion of proinflammatory cytokines. However, in a tumor cell line, TF-1, TL1A has been shown to induce caspase activity and apoptosis. These effects of TL1A are blocked by the secreted, soluble decoy receptor 3 (DcR3), also known as TR6 and TNFRSF6B, which compete with DR3 for binding to TL1A. Consistent with the observed in vitro activities, TL1A promotes ex vivo splenocyte expansion and enhances in vivo graft-versus-host-response.

References
  1. Migone, T.S. et al. (2002) Immunity 16:479.
Long Name
TNF-like 1
Entrez Gene IDs
9966 (Human); 326623 (Mouse)
Alternate Names
MGC129934; MGC129935; TL1A; TL1Vascular endothelial cell growth inhibitor; TNF superfamily ligand TL1A; TNFSF15; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand superfamily member 15; vascular endothelial growth inhibitor-192A; VEGI; VEGI192A; VEGITNF ligand-related molecule 1

Citations for Recombinant Human TL1A/TNFSF15 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Roles for ADAM17 in TNF-R1 Mediated Cell Death and Survival in Human U937 and Jurkat Cells
    Authors: J Fritsch, J Frankenhei, L Marischen, T Vadasz, A Troeger, S Rose-John, D Schmidt-Ar, W Schneider-
    Cells, 2021-11-10;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. TL1A-DR3 Plasma Levels Are Predictive of HIV-1 Disease Control, and DR3 Costimulation Boosts HIV-1-Specific T Cell Responses
    Authors: B Oriol-Tord, A Olvera, C Duran-Cast, A Llano, B Mothe, M Massanella, J Dalmau, C Ganoza, J Sanchez, ML Calle, B Clotet, J Martinez-P, E Negredo, J Blanco, D Hartigan-O, C Brander, M Ruiz-Riol
    J Immunol, 2020-11-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The Obesity-Susceptibility Gene TMEM18 Promotes Adipogenesis through Activation of PPARG
    Authors: K Landgraf, N Klöting, M Gericke, N Maixner, E Guiu-Jurad, M Scholz, AV Witte, F Beyer, JT Schwartze, M Lacher, A Villringer, P Kovacs, A Rudich, M Blüher, W Kiess, A Körner
    Cell Rep, 2020-10-20;33(3):108295.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. The TL1A-DR3 Axis Selectively Drives Effector Functions in Human MAIT Cells
    Authors: A Sattler, LG Thiel, AH Ruhm, N Souidi, M Seifert, G Herberth, K Kotsch
    J. Immunol., 2019-10-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions
    Authors: T Leng, HD Akther, CP Hackstein, K Powell, T King, M Friedrich, Z Christofor, S McCuaig, M Neyazi, CV Arancibia-, J Hagel, F Powrie, RS Peres, V Millar, D Ebner, R Lamichhane, J Ussher, TSC Hinks, E Marchi, C Willberg, P Klenerman
    Cell Rep, 2019-09-17;28(12):3077-3091.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Directed evolution of a soluble human DR3 receptor for the inhibition of TL1A induced cytokine secretion
    Authors: I Levin, M Zaretsky, A Aharoni
    PLoS ONE, 2017-03-09;12(3):e0173460.
    Species: Human, Yeast
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  7. PGE2 is a direct and robust mediator of anion/fluid secretion by human intestinal epithelial cells
    Sci Rep, 2016-11-09;6(0):36795.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. CX(3)CR1(+) mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.
    Authors: Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, Swaminath A, Bonneau R, Scherl E, Littman D
    J Exp Med, 2014-07-14;211(8):1571-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. TL1A increased the differentiation of peripheral Th17 in rheumatoid arthritis.
    Authors: Zhou M, Liu R, Su D, Feng X, Li X
    Cytokine, 2014-05-13;69(1):125-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Progranulin-derived Atsttrin directly binds to TNFRSF25 (DR3) and inhibits TNF-like ligand 1A (TL1A) activity.
    Authors: Liu, Cui, Li, Xing-Xia, Gao, Wei, Liu, Wen, Liu, De-Shan
    PLoS ONE, 2014-03-20;9(3):e92743.
    Species: Human, Mouse
    Sample Types: Protein, Whole Cells
    Applications: Binding Assay, Bioassay
  11. TL1A induces TCR independent IL-6 and TNF-alpha production and growth of PLZF(+) leukocytes.
    Authors: Reichwald K, Jorgensen T, Tougaard P, Skov S
    PLoS ONE, 2014-01-08;9(1):e85793.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The endothelial cell-produced antiangiogenic cytokine vascular endothelial growth inhibitor induces dendritic cell maturation.
    Authors: Tian F, Grimaldo S, Fujita M, Fugita M, Cutts J, Vujanovic NL, Li LY
    J. Immunol., 2007-09-15;179(6):3742-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human TL1A/TNFSF15 Protein

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Recombinant Human TL1A/TNFSF15 Protein
By Anonymous on 01/03/2020
Application: Binding assay/Protein-protein interaction
Reason for Rating: Recombinant human TL1A is bound by patented anti-TL1a antibodies via direct ELISA.

Recombinant Human TL1A/TNFSF15 Protein
By Vaishali Sarode on 02/06/2019
Application: Apoptosis assay