Recombinant Human DSCAM Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human DSCAM Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to bind biotinylated rhDSCAM in a functional ELISA with an apparent KD <10 nM.
Mouse myeloma cell line, NS0-derived human DSCAM protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
175 kDa
180-195 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: DSCAM

Down syndrome cell adhesion molecule (DSCAM) is a 220 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM, which maps to a Down syndrome region of chromosome 21q22.2-22.3, is synthesized as a 2012 amino acid (aa) precursor that contains a 17 aa signal sequence, a 1578 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 396 aa cytoplasmic tail (SwissProt #: O60469). The ECD contains ten Ig-like C2-type domains, six fibronectin type-III domains, and 16 potential sites for N-linked glycosylation. Splicing variants lead to a second, shorter isoform, which has a ten aa substitution for aa 1562 - 1571 in the longer isoform, and a deletion of residues corresponding to aa 1572 - 2012 in the longer isoform. Human mature DSCAM is 98% aa identical to mature mouse and rat DSCAM. Studies on mice have shown that DSCAM is expressed widely in the developing nervous system (1 - 2). More recent studies indicate that DSCAM plays an important role in neurite arborization, cell body spacing, and lamina-specific synaptic targeting in vertebrate retina (2 - 4). DSCAM signaling in vertebrates is not well understood, but it has been shown that DSCAM directly binds to Pak1 and stimulates Pak1 phosphorylation and activity (5). In addition, DSCAM activates both JNK and p38 MAP kinases, and expression of the cytoplasmic domain of DSCAM induces a morphological change in cultured cells that is JNK-dependent (5). Thus, it appears that DSCAM signals through Pak1 and functions in axon guidance. Furthermore, DSCAM, in collaboration with DCC, interacts with netrin-1, and is a receptor required for netrin-dependent commissural axon outgrowth and pathfinding (2, 6).

  1. Yamakawa, K. et al. (1998) Hum. Mol. Genet. 7:227.
  2. Liu, G. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:2951.
  3. Fuerst, P.G. et al. (2008) Nature 451:470.
  4. Yamagata, M. and J.R. Sanes, 2008, Nature 451:465.
  5. Li, W. and K.-L. Guan (2004) J. Biol. Chem. 279:32824.
  6. Ly, A. et al. (2008) Cell 133:11241.
Long Name
Down Syndrome Cell Adhesion Molecule
Entrez Gene IDs
1826 (Human); 13508 (Mouse)
Alternate Names
CHD2-42; CHD2-52; Down syndrome cell adhesion molecule; DSCAM; human CHD2-52 down syndrome cell adhesion molecule, 10CHD2


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