Recombinant Human Endorepellin Protein, CF

Catalog # Availability Size / Price Qty
2364-ER-050
Recombinant Human Endorepellin Protein, CF Binding Activity
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Product Details
Citations (2)
FAQs
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Reviews (2)

Recombinant Human Endorepellin Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF R1/Flt-1 (Catalog # 321-FL) is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Human Endorepellin/Perlecan that produces 50% of the optimal binding response is approximately 0.75-6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Endorepellin/Perlecan protein
Arg3684-Ser4391 (Ser4004Thr), with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Arg3684
Predicted Molecular Mass
76.7 kDa
SDS-PAGE
90-95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2364-ER

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Binding Activity View Larger

When Recombinant Human VEGFR1/Flt-1 Fc Chimera (Catalog # 321-FL) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human Endorepellin/Perlecan (Catalog # 2364-ER) binds with an ED50of 0.75-6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: Endorepellin/Perlecan

Endorepellin is an 80 kDa glycoprotein that is derived from the C-terminal end of the heparan sulfate proteoglycan, Perlecan. Perlecan itself is an approximately 850 kDa modular protein that contains multiple LDLR, EGF-like, Laminin-like, and immunoglobulin-like domains. Mouse Perlecan lacks several of the Ig-like domains found in the human protein (1-3). Endorepellin corresponds to domain V of Perlecan and consists of three Laminin G domains separated by four EGF-like domains (4). Human Endorepellin shares 89% amino acid sequence identity with mouse Endorepellin. A 26 kDa fragment of Endorepellin is known as LG3 and contains the third Laminin G-like domain of Endorepellin. LG3 can be released by Cathepsin L or BMP-1/Tolloid family mediated cleavage (4-6). Endorepellin binds to Integrin alpha 2/ beta 1, preventing the integrin-dependent adhesion of vascular endothelial cells (EC) to fibronectin and collagen I (7, 8). In contrast, this interaction enhances platelet adhesion to collagen as well as platelet aggregation and activation (9). Endorepellin additionally binds to VEGF R1 and VEGF R2 on EC (10). Its binding to VEGF R2 and Integrin alpha 2/ beta 1 on EC induces the association and down‑regulation of both proteins, followed by the inhibition of EC migration, tubulogenesis, secretion of VEGF, and the activation of multiple receptors involved in angiogenesis (4, 7, 8, 10, 11). Endorepellin also binds to Endostatin, resulting in a reduction of the anti-angiogenic activity of both proteins (4). It preferentially interacts with tumor vasculature and inhibits tumor growth and angiogenesis (8, 12).

References
  1. Whitelock, J.M. et al. (2008) Biochemistry 47:11174.
  2. Murdoch, A.D. et al. (1992) J. Biol. Chem. 267:8544.
  3. Noonan, D.M. et al. (1991) J. Biol. Chem. 266:22939.
  4. Mongiat, M. et al. (2003) J. Biol. Chem. 278:4238.
  5. Cailhier, J.-F. et al. (2008) J. Biol. Chem. 283:27220.
  6. Gonzalez, E.M. et al. (2005) J. Biol. Chem. 280:7080.
  7. Bix, G. et al. (2004) J. Cell Biol. 166:97.
  8. Woodall, B.P. et al. (2008) J. Biol. Chem. 283:2335.
  9. Bix, G. et al. (2007) Blood 109:3745.
  10. Goyal, A. et al. (2011) J. Biol. Chem. 286:25947.
  11. Nystrom, A. et al. (2009) Blood 114:4897.
  12. Bix, G. et al. (2006) J. Natl. Cancer Inst. 98:1634.
Entrez Gene IDs
3339 (Human); 15530 (Mouse)
Alternate Names
basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); Endorepellin; heparan sulfate proteoglycan 2; HSPG; HSPG2; perlecan proteoglycan; Perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1

Citations for Recombinant Human Endorepellin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Functional brain-specific microvessels from iPSC-derived human brain microvascular endothelial cells: the role of matrix composition on monolayer formation
    Authors: ME Katt, RM Linville, LN Mayo, ZS Xu, PC Searson
    Fluids Barriers CNS, 2018;15(1):7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet's Membrane Components
    Invest Ophthalmol Vis Sci, 2016;57(11):4599-605.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human Endorepellin Protein, CF

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Recombinant Human Endorepellin Protein, CF
By Anonymous on 03/13/2019
Application: CellProlif

Recombinant Human Endorepellin Protein, CF
By Anonymous on 05/15/2016
Application: Apoptosis assay
Reason for Rating: Dissolved easily and provided repeatable, expected results

Experiment was done to study effect of endorepellin on capillary-like tube formation of HUVEC cells