Recombinant Human EphA1 Fc Chimera Protein, CF Summary
Accession # AAA36747
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in MES, NaCl, PEG 3350, CHAPS and Imidazole.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
EphA1, also known as Eph and Esk, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1‑3). Mature mouse EphA1 consists of a 524 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (4). Within the ECD, human EphA1 shares 84% aa sequence identity with mouse and rat EphA1. EphA1 can form pH sensitive cis-homodimers on the cell surface (5). Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (6). Reverse signaling is propagated through the Ephrin ligand (7). EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (6, 8). It is additionally expressed on CD4+ T cells and a subpopulation of CD19+ B cells (9, 10). On CD4+ T cells, EphA1 interacts with EphA4, induces Tyr phosphorylation of PYK2, and promotes chemokine-induced chemotaxis (9, 10). EphA1 is up‑regulated or down‑regulated in a variety of human carcinomas and is implicated in tumor invasiveness (3, 7, 11). The region of Fibronectin including type I repeats #10‑12 interacts with EphA1, and this interaction plays a role in VEGF-dependent in vitro angiogenesis (12).
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- Mosch, B. et al. (2010) J. Oncol. Mar 10 Epub.
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- Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
- Coulthard, M.G. et al. (2001) Growth Factors 18:303.
- Aasheim, H.-C. et al. (2005) Blood 105:2869.
- Holen, H.L. et al. (2010) J. Leukoc. Biol. 87:1059.
- Dong, Y. et al. (2009) Mod. Pathol. 22:151.
- Masuda, J. et al. (2008) J. Biol. Chem. 283:13148.
Citations for Recombinant Human EphA1 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
The Journal of Biological Chemistry, 2022;0(0):101817.
Sample Types: Protein
Applications: ELISA Capture
ADAM12-cleaved ephrin-A1 contributes to lung metastasis.
Authors: Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y
Sample Types: In Vivo
Applications: In Vivo
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