Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein

Name: Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein
Brand: R&D Systems
SKU: BT602-025
Rating: 4 (1 reviews)

Catalog #: BT602
3 Citations 1 Review Datasheet / COA / SDS

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BT602-025

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Citations (3)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse EphA2 Fc Chimera (Catalog # 639-A2) at 2 µg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse Ephrin‑A1 Fc Chimera with a linear range of 0.078‑5 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein

Mouse Ephrin-A1 (Asp19-Ser182) Accession # P52793	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

P52793

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46.8 kDa (monomer)

SDS-PAGE

50-55 kDa, reducing conditions

Product Datasheets

BT602

Product Datasheet

COA

SDS

BT602

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL with sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Ephrin-A1

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21‑25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10‑12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).

References

Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
Easty, D.J. et al. (1995) Cancer Res. 55:2528.
Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
Aasheim, H.-C. et al. (2005) Blood 105:2869.
Ogawa, K. et al. (2000) Oncogene 19:6043.
Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
Pandey, A. et al. (1995) Science 268:567.
Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

Entrez Gene IDs

1942 (Human); 13636 (Mouse)

Alternate Names

B61; ECKLG; EFL1; EFL-1; EFNA1; EPH-related receptor tyrosine kinase ligand 1; EphrinA1; Ephrin-A1; EPLG1TNF alpha-induced protein 4; Immediate early response protein B61; LERK1; LERK1LERK-1; ligand of eph-related kinase 1; TNFAIP4B61; Tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
Authors: M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara
Nat Commun, 2017-06-06;8(0):15729.
Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay
Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.
Authors: Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E
Mol Biol Cell, 2016-07-06;27(17):2757-70.
Species: Human
Sample Types: Whole Cells
Applications: Immunoprecipitation
Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
PLoS ONE, 2011-03-30;6(3):e18128.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay