Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein Summary
Optimal dilutions should be determined by each laboratory for each application.
Accession # P52793
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.|
Reconstitute at 100 μg/mL with sterile PBS.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21‑25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10‑12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).
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- Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
- Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
- Easty, D.J. et al. (1995) Cancer Res. 55:2528.
- Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
- Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
- Aasheim, H.-C. et al. (2005) Blood 105:2869.
- Ogawa, K. et al. (2000) Oncogene 19:6043.
- Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
- Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
- Pandey, A. et al. (1995) Science 268:567.
- Magal, E. et al. (1996) J. Neurosci. Res. 43:735.
Citations for Recombinant Mouse Ephrin-A1 Fc Chimera Biotinylated Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2
Authors: M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara
Nat Commun, 2017;8(0):15729.
Sample Types: Recombinant Protein
Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation.
Authors: Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M
PLoS ONE, 2011;6(3):e18128.
Sample Types: Recombinant Protein
Applications: Binding Assay
Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.
Authors: Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E
Mol Biol Cell, 0;27(17):2757-70.
Sample Types: Whole Cells
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