Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10170-SG-050
Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF Bioactivity
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Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.8-4.8 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human epsilon-Sarcoglycan protein
Human epsilon-Sarcoglycan
(Asp47-Phe317)
Accession # O43556-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp47
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
58 kDa
SDS-PAGE
57-71 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10170-SG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Human epsilon‑Sarcoglycan Fc Chimera (Catalog #10170-SG) supports the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50for this effect is 0.8-4.8 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human epsilon-Sarcoglycan Fc Chimera (Catalog # 10170-SG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-71 kDa and 110-140 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: epsilon-Sarcoglycan

SGCE, or Epsilon-Sarcoglycan, is a type 1 transmembrane glycoprotein which was found as a homologue of alpha -sarcoglycan (1, 2). Sarcoglycans are part of the dystrophin-associated glycoprotein complex (DGC). This multiprotein complex connects the actin cytoskeleton to the extracellular matrix in cardiac and skeletal muscles (3, 4). The sarcogylcan complex is a subcomplex within the DGC and is composed of several muscle-specific forms. alpha, beta, gamma and δ-sarcoglycans are expressed predominantly in striated and smooth muscles while epsilon -Sarcoglycan (SGCE) is expressed in a wide range of tissues, with the highest levels in heart and lung (1-2, 5). Mutations in the human SGCE gene have been shown to be associated with myoclonus-dystonia (6). Human SGCE is synthesized as a 437 amino acid (aa) protein that includes a 317 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain. Within the ECD, human SGCE shares 95% and 66% aa sequence identity the mouse and rat SGCE, respectively. There are multiple isoforms of SGCE protein present in humans. SGCE exon 11b isoform is differentially expressed in the brain (1). Knockout mutations in this isoform result in psychiatric diseases (7).

References
  1. Ettinger, A.J. et al. (1997) J. Biol. Chem. 272:32534.
  2. McNally, E.M. et al. (1998) FEBS Lett. 422:27.
  3. Blake, D.J. et al. (2002) Physiol. Rev. 82:291.
  4. Xiao, J. et al. (2017) Neurobiol. Dis. 98:52.
  5. Nishiyama, A. et al. (2004) Brain Res. Mol. Brain Res. 125:1.
  6. Zimprich, A. et al. (2001) Nat. Genet. 29:66.
  7. Peall. K.J. et al. (2013) Brain. 136:294.
Entrez Gene IDs
8910 (Human); 20392 (Mouse); 432360 (Rat)
Alternate Names
dystonia 11, myoclonic; DYT11; epsilon-Sarcoglycan; Epsilon-SG; ESG; Sarcoglycan epsilon; sarcoglycan, epsilon; SGCE

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