Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF Summary
Accession # O43556-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human epsilon‑Sarcoglycan Fc Chimera (Catalog #10170-SG) supports the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50for this effect is 0.8-4.8 μg/mL.
2 μg/lane of Recombinant Human epsilon-Sarcoglycan Fc Chimera (Catalog # 10170-SG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-71 kDa and 110-140 kDa, respectively.
SGCE, or Epsilon-Sarcoglycan, is a type 1 transmembrane glycoprotein which was found as a homologue of alpha -sarcoglycan (1, 2). Sarcoglycans are part of the dystrophin-associated glycoprotein complex (DGC). This multiprotein complex connects the actin cytoskeleton to the extracellular matrix in cardiac and skeletal muscles (3, 4). The sarcogylcan complex is a subcomplex within the DGC and is composed of several muscle-specific forms. alpha, beta, gamma and δ-sarcoglycans are expressed predominantly in striated and smooth muscles while epsilon -Sarcoglycan (SGCE) is expressed in a wide range of tissues, with the highest levels in heart and lung (1-2, 5). Mutations in the human SGCE gene have been shown to be associated with myoclonus-dystonia (6). Human SGCE is synthesized as a 437 amino acid (aa) protein that includes a 317 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain. Within the ECD, human SGCE shares 95% and 66% aa sequence identity the mouse and rat SGCE, respectively. There are multiple isoforms of SGCE protein present in humans. SGCE exon 11b isoform is differentially expressed in the brain (1). Knockout mutations in this isoform result in psychiatric diseases (7).
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- Xiao, J. et al. (2017) Neurobiol. Dis. 98:52.
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- Peall. K.J. et al. (2013) Brain. 136:294.
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