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Recombinant Human ErbB2/Her2 His-tag Protein, CF

R&D Systems | Catalog # 10126-ER

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human ErbB2/Her2 His-tag Protein (10126-ER)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P04626-1

Applications

Bioactivity
View all ErbB2/Her2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ErbB2/Her2 protein
Ser22-Thr652, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

70 kDa

SDS-PAGE

93-108 kDa, under reducing conditions

Activity

Measured by its ability to block anti-ErbB2 mediated inhibition of SK‑BR‑3 human breast cancer cell proliferation. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875.
The ED50 for this effect is 0.3-1.2 μg/mL in the presence of 0.6 µg/mL Human ErbB2/Her2 Antibody (Trastuzumab, Catalog # MAB9589).

Scientific Data Images for Recombinant Human ErbB2/Her2 His-tag Protein, CF

Recombinant Human ErbB2/Her2 His-tag Protein SEC-MALS.

Recombinant Human ErbB2/Her2 His-tag Protein (Catalog # 10126-ER) has a molecular weight (MW) of 80-88 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human ErbB2/Her2 His-tag Protein Bioactivity

Recombinant Human ErbB2/Her2 His-tag Protein Bioactivity

Recombinant Human ErbB2/Her2 His-tag (Catalog # 10126-ER) blocks anti-hErbB2 antibody (Trastuzumab, Catalog # MAB9589) mediated inhibition of the SK-BR-3 human breast cancer cell line proliferation. The ED50 is 0.3-1.2 µg/mL in the presence of 0.6 µg/mL antibody (Trastuzumab, Catalog # MAB9589).

Formulation, Preparation, and Storage

10126-ER
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I transmembrane glycoprotein member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factors. It is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among the ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Human ErbB2 consists of 1255 amino acids (aa) including a 22 aa signal sequence, a 630 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 580 aa cytoplasmic domain.  Within the ECD, human ErbB2 shares and 86% aa sequence identity with mouse and rat ErbB2.  Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (1-10).

References

  1. Coussens, L. et al. (1985) Science 230:1132.
  2. Yamamoto, T. et al. (1986) Nature 319:230.
  3. Kanai, Y. et al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

UniProt

P04626-1

Additional ErbB2/Her2 Products

Product Documents for Recombinant Human ErbB2/Her2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human ErbB2/Her2 His-tag Protein, CF

For research use only

Citations for Recombinant Human ErbB2/Her2 His-tag Protein, CF

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