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Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

R&D Systems | Catalog # MAB9589

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Bacteria - Staphylococcus, Bacteria - Streptococcus

Applications

Validated:

Inhibition of Cell Growth, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation, ELISA Detection, Neutralization Control

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu5
View all ErbB2/Her2 Primary Antibodies »

Product Specifications

Immunogen

Human ErbB2/Her2

Specificity

Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Detection of ErbB2/Her2 antibody in MCF-7 human cell line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.

MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589, filled histogram) or Human IgG Control Antibody (1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
Detection of ErbB2/Her2 antibody in MDA-MB-453 human cell line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in MDA-MB-453 human cell line by Flow Cytometry.

MDA-MB-453 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589, filled histogram) or Human IgG Control Antibody (1-001-A, open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.
Inhibition of Cell Proliferation by ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody.

Inhibition of Cell Proliferation by ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody.

Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9589) inhibits proliferation in the SK-BR-3 human breast cancer cell line as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 15-75 ng/mL.

NKs cells are potent killers in ADCC assays with SKOV-3 or Raji cells (A, B)

ADCC assays with SKOV-3 and Raji targets. NK cells were cultured as described in the adjacent figure for 13 days. Prior to setting up the ADCC assay, CD56+CD16+ levels were ascertained to be >30% on NKs using Human Fc gamma RIII (CD16) PerCP-conjugated Antibody (FAB2546C); in our hands, this is the minimum level required for killing via ADCC. Target cells were labeled with Mito Mark Green (SKOV-3 or Raji), and Effectors/NKs were labeled with Janelia Fluor 646 as described (Figure 1). (A) SKOV-3 cells were preincubated with 5 ug/mL Anti-Her2/Trastuzumab (Catalog # MAB9589); (B) Raji cells were preincubated with 10 ug/mL Anti-CD20/Rituximab (MAB9630), both for 30 minutes at room temperature prior to setting up the ADCC assay with the NKs. 0.5:1 E:T ratios were used for the SKOV-3/Trastuzumab model, and NKs and SKOV-3 cells were co-incubated for 2 hours at 37°C. 2:1 E:T ratios were used for the Raji/Rituximab model, and NKs and Raji cells were co-incubated for 2.5 hours at 37°C. In both models, the cells were harvested, stained with antibodies for Human CD3 Alexa Fluor 405 and Human CD56 Alexa Fluor 700, and analyzed by flow cytometry. Importantly, non-NKs (JF 646-CD56- cells) are shown in the Mito Mark dye graphs that depict killing. These results are representative of 3+ experiments.

Applications for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: MCF-7 Human cell line and MDA-MB-453 human breast cancer cell line

Inhibition of Cell Growth

Measured by its ability to inhibit proliferation in the SK‑BR‑3 human breast cancer cell line. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is typically 15-75 ng/mL.

Reviewed Applications

Read 3 reviews rated 4 using MAB9589 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ErbB2/Her2

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product constitutes the immunogen used for the anti-Idiotype catalog numbers MAB9547 and MAB95471. The biotinylated antibody (catalog number FAB9589B) can be used in flow cytometry or various assay formats to measure Her2 expression and can be detected by conjugated streptavidin.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

Additional ErbB2/Her2 Products

Product Documents for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

For research use only

Citations for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

Customer Reviews for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody (3)

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  • Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody
    Name: Anonymous
    Application: Protein:Protein interaction
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 09/15/2021
    Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody MAB9589
  • Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: human HEK293T cells
    Species: Human
    Verified Customer | Posted 06/17/2021
    Transfected cell line with HER2. Cells grown on slide, stained for HER2 (green) and counterstained with DAPI.
    Immunofluorescence on 4% PFA fixed cells after Triton X-100 permeabilization. The staining was done on Human HEK293 cell line that was transfected with a viral vector to express HER2.
    Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody MAB9589
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody
    Name: Anonymous
    Application: Protein to protein interaction
    Sample Tested: Serum
    Species: Human and Cynomolgus Monkey
    Verified Customer | Posted 10/19/2020
    Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody MAB9589

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FAQs for Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Antibody

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  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is an isotype control available for human IgG Biosimilar Antibodies?

    A: Unfortunately, we do not offer isotype controls for Biosimilar antibodies at this time. We do have a polyclonal Normal Human IgG Control, Catalog # 1-001-A. 

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is an isotype control available for human IgG Biosimilar Antibodies?

    A: Unfortunately, we do not offer isotype controls for Biosimilar antibodies at this time. We do have a polyclonal Normal Human IgG Control, Catalog # 1-001-A. 

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  • Q: Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    A: This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

  • Q: Is an isotype control available for human IgG Biosimilar Antibodies?

    A: Unfortunately, we do not offer isotype controls for Biosimilar antibodies at this time. We do have a polyclonal Normal Human IgG Control, Catalog # 1-001-A. 

  • Q: What is a Biosimilar antibody?

    A: A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

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