Recombinant Human ESAM Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2688-EC-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human ESAM Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce ERK phosphorylation in BaF3 mouse pro‑B cells transfected with human ESAM. Stimulation of 2.5 x 105 serum-starved hESAM transfected BaF3 cells with 25 µg Recombinant Human ESAM Fc Chimera (Catalog # 2688-EC) for 30 minutes at 37° C leads to ERK phosphorylation.
Source
Mouse myeloma cell line, NS0-derived human ESAM protein
Human ESAM
(Gln30 - Ala247)
Accession # Q96AP7
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln30 predicted
Predicted Molecular Mass
50.3 kDa (monomer)
SDS-PAGE
67-75 kDa, reducing conditions

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2688-EC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2688-EC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: ESAM

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

References
  1. Hirata, K-I, et al. (2001) J. Biol. Chem. 276:16223.
  2. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294.
  3. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121.
  4. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.
Long Name
Endothelial Cell Adhesion Molecule
Entrez Gene IDs
90952 (Human); 69524 (Mouse)
Alternate Names
2310008D05Rik; endothelial cell adhesion molecule; endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m

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