Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein

Newer Version Available: 7398-FS
NEW
New rhFas! HEK293 expressed; ~2 fold better activity; Lower endotoxin specification; No His tag!

Carrier Free

Catalog # Availability Size / Price Qty
326-FS-050/CF

With Carrier

Catalog # Availability Size / Price Qty
326-FS-050
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (5)
FAQs
Reviews (1)

Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759. The ED50 for this effect is 0.01‑0.04 µg/mL in the presence of 2 ng/mL recombinant human Fas Ligand.
Source
Mouse myeloma cell line, NS0-derived human Fas/TNFRSF6/CD95 protein
Human Fas
(Arg17-Asn173)
Accession # Q5T9P3
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
No results obtained: Arg17 predicted
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45 kDa (monomer)

Product Datasheets

326-FS (with carrier)

326-FS/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

326-FS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

326-FS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fas/TNFRSF6/CD95

Fas, also known as APO‑1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6 (1-3). The 335 amino acid (aa) human Fas includes a 25 aa signal peptide, a 148 aa extracellular domain (ECD) with three cysteine-rich TNFR repeats, a 17 aa transmembrane sequence, and a 145 aa cytoplasmic domain containing a death domain (DD), which is required for transducing apoptotic signals (4). Mature human Fas ECD shares 55%, 58%, 62%, 63%, and 64% aa sequence identity with mouse, rat, feline, bovine and porcine Fas, respectively. A human Fas isoform of 314 aa that lacks the transmembrane sequence is secreted by resting lymphocytes, while isoforms of 149, 132, 103 and 86 aa that also lack the DD and show substitutions for parts of the TNFR repeats are less prominently expressed (4-6). All appear to block the extrinsic apoptosis pathway induced by the Fas ligand (FasL, TNFSF6), a type II transmembrane protein of the TNF family that can be expressed on activated T‑lymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form (2, 6). Engagement of FAS induces oligomerization of preformed Fas trimers (1, 2). The activated receptor recruits the adaptor molecule FADD to form the Death‑Inducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade (7). Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activation‑induced death of T lymphocytes that terminates the immune reaction (1, 2, 8). In immune‑privileged tissues, infiltrating Fas‑bearing lymphocytes and inflammatory cells are killed by FasL engagement (9). Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (1-3). The Fas pathway also appears to cross‑communicate with the BIM (mitochondrial/intrinsic) apoptosis pathway (1).

References
  1. Bouillet, P. and L.A. O’Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  4. SwissProt accession P25445.
  5. Liu, C. et al. (1995) Biochem. J. 310:957.
  6. Papoff, G. et al. (1996) J. Immunol. 156:4622.
  7. Thorburn, A. (2003) Cellular Signaling 16:139.
  8. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
  9. Ferguson, T.A. and T.S Griffith (2006) Immunol. Rev. 213:228.
Long Name
Fibroblast-associated
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Citations for Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Defective Regulation of Membrane TNF&alpha Expression in Dendritic Cells of Glioblastoma Patients Leads to the Impairment of Cytotoxic Activity against Autologous Tumor Cells
    Authors: T Tyrinova, O Leplina, S Mishinov, M Tikhonova, E Dolgova, A Proskurina, V Stupack, S Bogachev, A Ostanin, E Chernykh
    Int J Mol Sci, 2020;21(8):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli
    Authors: AE Dodi, IO Ajayi, C Chang, M Beard, SL Ashley, SK Huang, VJ Thannickal, DJ Tschumperl, TH Sisson, JC Horowitz
    Respir. Res., 2018;19(1):91.
    Applications: ELISA (Standard)
  3. IFN-beta induces caspase-mediated apoptosis by disrupting mitochondria in human advanced stage colon cancer cell lines.
    Authors: Juang SH, Wei SJ, Hung YM, Hsu CY, Yang DM, Liu KJ, Chen WS, Yang WK
    J. Interferon Cytokine Res., 2004;24(4):231-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IFN-beta induces caspase-mediated apoptosis by disrupting mitochondria in human advanced stage colon cancer cell lines.
    Authors: Juang SH, Wei SJ, Hung YM, Hsu CY, Yang DM, Liu KJ, Chen WS, Yang WK
    J. Interferon Cytokine Res., 2004;24(4):231-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Functional and phenotypic changes in circulating lymphocytes from hospitalized zambian children with measles.
    Authors: Ryon JJ, Moss WJ, Monze M, Griffin DE
    Clin. Diagn. Lab. Immunol., 2002;9(5):994-1003.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein
By Anonymous on 11/16/2017
Application: Immunoassay Standard