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Recombinant Human Fc gamma RIIA/CD32a (H167) Protein, CF

R&D Systems | Catalog # 9595-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Fc gamma RIIA/CD32a (H167) Protein (9595-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P12318-1

Applications

Bioactivity
View all Fc gamma RIIA/CD32a Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Fc gamma RIIA/CD32a protein
Gln34-Ile218, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala36 identified. Gln34 inferred from enzymatic pyroglutamate treatment revealing Ala35 

Predicted Molecular Mass

22 kDa

SDS-PAGE

25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Fc gamma  RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25‑1.25 μg/mL.

Scientific Data Images for Recombinant Human Fc gamma RIIA/CD32a (H167) Protein, CF

Recombinant Human Fc gamma RIIA/CD32a (H167) Protein Bioactivity

Recombinant Human Fc gamma RIIA/CD32a (H167) Protein Bioactivity

When Recombinant Human Fc gamma RIIA/CD32a (H167) (Catalog # 9595-CD) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25-1.25 μg/mL.

Formulation, Preparation, and Storage

9595-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, and Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be co-expressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Chi, M. et al. (2002) J. Immunol. 168:1413.
  5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

Long Name

Fc gamma Receptor II A

Alternate Names

CD32a, FCGR2A, FcgRIIA, FCRIIA

Entrez Gene IDs

2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)

Gene Symbol

FCGR2A

UniProt

P12318-1

Additional Fc gamma RIIA/CD32a Products

Product Documents for Recombinant Human Fc gamma RIIA/CD32a (H167) Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fc gamma RIIA/CD32a (H167) Protein, CF

For research use only

Citations for Recombinant Human Fc gamma RIIA/CD32a (H167) Protein, CF

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