Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF

(6 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind human IgG with an estimated Kd <200 nM.
  • Source
    Mouse myeloma cell line, NS0-derived Ala46-Pro217, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala46
  • Predicted Molecular Mass
    21 kDa
  • SDS-PAGE
    25-35 kDa, reducing conditions
1875-CD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Binding Activity
Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD) binds human IgG with an estimated Kd 200 nM.
1 μg/lane of Recombinant Human Fc gamma  RIIB/C (CD32b/c) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing multiple bands at 27-35 kDa.
Background: Fc gamma RIIB/C (CD32b/c)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

  • References:
    1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
    2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
    3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
    4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
    5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  • Long Name:
    Fc gamma Receptor IIB/C
  • Entrez Gene IDs:
    2213 (Human); 14130 (Mouse)
  • Alternate Names:
    Fc gamma RIIB/C (CD32b/c)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Enzymatic inactivation of endogenous IgG by IdeS enhances�therapeutic antibody efficacy
    Authors: S Järnum, A Runström, R Bockermann, L Winstedt, M Crispin, C Kjellman
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Type: Antibody
    Application: Bioassay
  2. GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins.
    Authors: Meuris L, Santens F, Elson G, Festjens N, Boone M, Dos Santos A, Devos S, Rousseau F, Plets E, Houthuys E, Malinge P, Magistrelli G, Cons L, Chatel L, Devreese B, Callewaert N
    Nat Biotechnol, 2014;32(5):485-9.
    Species: Human
    Sample Type: Protein
    Application: Bioassay
  3. Engineering of a novel anti-CD40L domain antibody for treatment of autoimmune diseases.
    Authors: Xie J, Yamniuk A, Borowski V, Kuhn R, Susulic V, Rex-Rabe S, Yang X, Zhou X, Zhang Y, Gillooly K, Brosius R, Ravishankar R, Waggie K, Mink K, Price L, Rehfuss R, Tamura J, An Y, Cheng L, Abramczyk B, Ignatovich O, Drew P, Grant S, Bryson J, Suchard S, Salter-Cid L, Nadler S, Suri A
    J Immunol, 2014;192(9):4083-92.
    Species: Human
    Sample Type: Whole Tissue
    Application: Bioassay
  4. Increasing FcgammaRIIa affinity of an FcgammaRIII-optimized anti-EGFR antibody restores neutrophil-mediated cytotoxicity.
    Authors: Derer S, Glorius P, Schlaeth M, Lohse S, Klausz K, Muchhal U, Desjarlais J, Humpe A, Valerius T, Peipp M
    MAbs, 2014;6(2):409-21.
    Species: N/A
    Sample Type: Chip
    Application: Surface Plasmon Resonance
  5. Development and characterisation of monoclonal antibodies specific for the murine inhibitory FcgammaRIIB (CD32B).
    Eur J Immunol, 2012;42(8):2109-20.
    Species: N/A
    Sample Type: Antibody
    Application: Surface Plasmon Resonance
  6. Optimization of antibody binding to FcgammaRIIa enhances macrophage phagocytosis of tumor cells.
    Authors: Richards JO, Karki S, Lazar GA, Chen H, Dang W, Desjarlais JR
    Mol. Cancer Ther., 2008;7(8):2517-27.
    Species: Human
    Sample Type: Antibody
    Application: Surface Plasmon Resonance
Primary Antibodies
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His Tag Antibody

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Histidine-tagged Protein Purification Resin

IP999 1
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