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Recombinant Human FCRN Protein, CF

R&D Systems | Catalog # 8639-FC

Analyzed by SEC-MALS. Non-covalently associated with endogenous beta 2-Microglobulin
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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human FCRN Protein (8639-FC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P55899

Structure / Form

Non-covalently associated with beta 2 Microglobulin from HEK293 cells.

Applications

Bioactivity
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Why choose R&D Systems FCRN Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human FCRN protein
Ala24-Ser297, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala24 (FCRN), Ile21 ( beta 2 Microglobulin)

Predicted Molecular Mass

31 kDa (FCRN), 10 kDa ( beta 2 Microglobulin)

SDS-PAGE

30-36 kDa (FCRN), 10 kDa ( beta 2 Microglobulin)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FCRN is immobilized at 2 µg/mL (100 µL/well), the concentration of biotinylated human IgG that produces 50% of the optimal binding response is approximately 2-10 μg/mL.

Reviewed Applications

Read 3 reviews rated 5 using 8639-FC in the following applications:

Scientific Data Images for Recombinant Human FCRN Protein, CF

Recombinant Human FCRN Protein SEC-MALS.

Recombinant Human FCRN (Catalog # 8639-FC) has a molecular weight (MW) of 45.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing dose-dependent bioactivity of human FCRN protein

Bioactivity of human FCRN

When recombinant human FCRN (8639-FC) is immobilized at 2 µg/mL (100 µL/well), the concentration of biotinylated human IgG that produces 50% of the optimal binding response is approximately 2-10 μg/mL.

Formulation, Preparation, and Storage

8639-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FCRN

The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells (1, 2). Mature human FCRN consists of a 274 amino acid (aa) extracellular domain (ECD) with two N-terminal alpha domains, one alpha 3/immunoglobulin-like domain, a 23 aa transmembrane segment, and a 44 aa cytoplasmic domain (3). Within the ECD, human FCRN shares 68% aa sequence identity with mouse and rat FCRN. Mouse FCRN binds with high affinity to IgG from mouse, human, rat, rabbit, guinea pig, bovine, and sheep, while human FCRN binds IgG with significantly lower affinity and is much more restricted in terms of species recognition (4). It does not bind the structurally related chicken IgY (5). FCRN additionally binds to albumin, and both it and IgG are bound at pH 5.0 but not at pH 8.0 (3, 6). FCRN associates noncovalently with beta 2-Microglobulin, and this interaction is important for the intracellular trafficking of FCRN (7-10). FCRN cycles between the plasma membrane and acidified intracellular compartments of endothelial cells and epithelial cells (5, 8). It binds endocytosed IgG and albumin in the low pH vesicles and transports them to the plasma membrane for extracellular release at higher pH. This protects IgG and albumin from lysosomal degradation and helps maintain the circulating levels of both proteins (5, 6). This mechanism is involved in the bidirectional transport of IgG across epithelial and endothelial barriers including neonatal IgG absorption in the intestine and fetal uptake of maternal antibodies through the placenta (5, 8, 11, 12). In the kidney, FCRN recycles albumin to the serum but removes IgG from the glomular basement membrane and promotes its excretion into the urine (13, 14). FCRN is also expressed in neutrophils and myeloid antigen presenting cells (7, 15, 16). It can enhance IgG-mediated phagocytosis and antigen presentation by these cells, but it promotes the degradation of opsonizing IgG rather than returning it to the circulation (15, 16).

References

  1. Baker, K. et al. (2009) Semin. Immunopathol. 31:223.
  2. Baker, K. et al. (2014) Front. Immunol. 5:408.
  3. Story, C.M. et al. (1994) J. Exp. Med. 180:2377.
  4. Ober, R.J. et al. (2001) Int. Immunol. 13:1551.
  5. Dickinson, B.L. et al. (1999) J. Clin. Invest. 104:903.
  6. Chaudhury, C. et al. (2003) J. Exp. Med. 197:315.
  7. Simister, N.E. and K.E. Mostov (1989) Nature 337:184.
  8. Kobayashi, N. et al. (2002) Am. J. Physiol. Renal Physiol. 282:F358.
  9. Praetor, A. and W. Hunziker (2002) J. Cell Sci. 115:2389.
  10. Zhu, X. et al. (2001) J. Immunol. 166:3266.
  11. Spiekermann, G.M. et al. (2002) J. Exp. Med. 196:303.
  12. Firan, M. et al. (2001) Int. Immunol. 13:993.
  13. Sarav, M. et al. (2009) J. Am. Soc. Nephrol. 20:1941.
  14. Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967.
  15. Vidarsson, G. et al. (2006) Blood 108:3573.
  16. Qiao, S.-W. et al. (2008) Proc. Natl. Acad. Sci. 105:9337.

Long Name

Neonatal Fc Receptor

Alternate Names

FCGRT

Entrez Gene IDs

2217 (Human); 14132 (Mouse); 29558 (Rat); 102128913 (Cynomolgus Monkey)

Gene Symbol

FCGRT

UniProt

P55899

Additional FCRN Products

Product Documents for Recombinant Human FCRN Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human FCRN Protein, CF

For research use only

Related Research Areas

Fc Receptors

Citations for Recombinant Human FCRN Protein, CF

Customer Reviews for Recombinant Human FCRN Protein, CF (3)

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  • Recombinant Human FCRN Protein, CF
    Name: Jenna Nguyen
    Application: Cell Proliferation
    Verified Customer | Posted 05/07/2019
  • Recombinant Human FCRN Protein, CF
    Name: Anonymous
    Application: Biomarker discovery
    Verified Customer | Posted 09/05/2018
  • Recombinant Human FCRN Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/06/2017
    Recombinant Human FCRN Protein, CF 8639-FC

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