Recombinant Human FGF-21 Protein

Carrier Free

Catalog # Availability Size / Price Qty
2539-FG-025/CF

With Carrier

Catalog # Availability Size / Price Qty
2539-FG-025
Recombinant Human FGF-21 Protein Bioactivity
2 Images
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Recombinant Human FGF-21 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.06-0.4 µg/mL in the presence of Recombinant Mouse Klotho  beta (Catalog # 2619-KB).
Source
E. coli-derived human FGF-21 protein
His29-Ser209, with a N-terminal 5-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
20.2 kDa
SDS-PAGE
24 kDa, reducing conditions

Product Datasheets

2539-FG (with carrier)

2539-FG/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2539-FG

Formulation Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2539-FG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MES, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human FGF-21 Protein Bioactivity View Larger

Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates cell proliferation of the BaF3 mouse pro-B cell line transfected with human FGF RIIIc. The ED50 for this effect is 0.06-0.4 µg/mL in the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB).

SDS-PAGE Recombinant Human FGF-21 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human FGF-21 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-21

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules (3). The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region (4). Notably, FGF-21, as well as FGF-19 show limited binding to heparin (4). One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form (5). Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells (4, 6). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction (7, 8, 9). FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex (3, 7, 10, 11). This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation (11, 12).

References
  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Huang, X. et al. (2006) Mol. Carcinog. 45:934.
  4. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  5. GenBank Accession #: EAW52401 (2006).
  6. Ford, A.M. et al. (2005) J. Clin. Invest. 115:1627.
  7. Moore, D. D. (2007) Science 316:1436.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
  9. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
  10. Lundasen, T. et al. (2007) Biochem. Biophys. Res. Commun. 360:437.
  11. Badman, M.K. et al. (2007) Cell Metab. 5:426.
  12. Inagaki, T. et al. (2007) Cell Metab. 5:415.
Long Name
Fibroblast Growth Factor 21
Entrez Gene IDs
26291 (Human); 56636 (Mouse)
Alternate Names
FGF21; FGF-21; fibroblast growth factor 21

Citations for Recombinant Human FGF-21 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Circulating alphaKlotho influences phosphate handling by controlling FGF23 production.
    Authors: Smith R
    J. Clin. Invest., 2102;122(12):4710-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Peripherally derived FGF21 promotes remyelination in the central nervous system
    Authors: M Kuroda, R Muramatsu, N Maedera, Y Koyama, M Hamaguchi, H Fujimura, M Yoshida, M Konishi, N Itoh, H Mochizuki, T Yamashita
    J. Clin. Invest., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Unliganded fibroblast growth factor receptor 1 forms density-independent dimers.
    Authors: Comps-Agrar L, Dunshee D, Eaton D, Sonoda J
    J Biol Chem, 2015;290(40):24166-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. FGF19 Regulates Cell Proliferation, Glucose and Bile Acid Metabolism via FGFR4-Dependent and Independent Pathways.
    Authors: Wu AL, Coulter S, Liddle C, Wong A, Eastham-Anderson J, French DM, Peterson AS, Sonoda J
    PLoS ONE, 2011;6(3):e17868.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010;177(5):2379-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Human FGF-21 Protein

Average Rating: 4.7 (Based on 3 Reviews)

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Recombinant Human FGF-21 Protein
By Anonymous on 05/14/2021
Application: Immunoassay Standard

Recombinant Human FGF-21 Protein
By Jessica Fernando on 10/23/2017
Application: Standards

By SUNEET MEHROTRA on 04/26/2017