Recombinant Mouse Klotho beta Protein, CF

(1 citations)   
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 µg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG).
  • Source
    Mouse myeloma cell line, NS0-derived Phe53-Leu995, with a C-terminal 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Phe53
  • Predicted Molecular Mass
    110.2 kDa
  • SDS-PAGE
    140 kDa, reducing conditions
2619-KB
 
Formulation Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Data Images
Recombinant Mouse Klotho beta (Catalog # 2619-KB) stimulates cell proliferation of the BaF3 mouse pro-B cell line transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 μg/mL in the presence of Recombinant Human FGF‑21 (Catalog # 2539-FG).
1 μg/lane of Recombinant Mouse Klotho beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 135 kDa.
Background: Klotho beta

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF-19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-4). Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes, which allows FGF-21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (2-3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho  beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

  • References:
    1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
    2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
    3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
    4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
    5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
    6. Chang, Q. et al. (2005) Science 310:490.
    7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  • Entrez Gene IDs:
    152831 (Human); 83379 (Mouse); 289625 (Rat)
  • Alternate Names:
    betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Different roles of N- and C- termini in the functional activity of FGF21.
    Authors: Micanovic R, Raches DW, Dunbar JD, Driver DA, Bina HA, Dickinson CD, Kharitonenkov A
    J. Cell. Physiol., 2009;219(2):227-34.
    Species: Mouse
    Sample Type: Peptide
    Application: Surface Plasmon Resonance
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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Recombinant Proteins
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Recombinant Human FGF-23 Protein, CF

BA 2604-FG/CF 10
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Recombinant Human FGF-23 Protein

BA 2604-FG 10
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