Recombinant Mouse Klotho beta Protein, CF

Catalog # Availability Size / Price Qty
2619-KB-050
Recombinant Mouse Klotho beta Protein Bioactivity
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Recombinant Mouse Klotho beta Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 µg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG) and Heparin.
Source
Mouse myeloma cell line, NS0-derived mouse Klotho beta protein
Phe53-Leu995, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Phe53
Predicted Molecular Mass
110.2 kDa
SDS-PAGE
140 kDa, reducing conditions

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2619-KB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2619-KB

Formulation Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Mouse Klotho beta Protein Bioactivity View Larger

Recombinant Mouse Klotho beta (Catalog # 2619-KB) stimulates cell proliferation of the BaF3 mouse pro-B cell line transfected with human FGF RIIIc. The ED50 for this effect is 0.2-1 µg/mL in the presence of Recombinant Human FGF-21 (Catalog # 2539-FG) and Heparin.

SDS-PAGE Recombinant Mouse Klotho beta Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Klotho beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 135 kDa.

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Background: Klotho beta

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF-19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2-4). Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes, which allows FGF-21 to stimulate GLUT1 expression, up-regulating adipocyte insulin-dependent glucose uptake (2-3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho  beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.

References
  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  6. Chang, Q. et al. (2005) Science 310:490.
  7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
Entrez Gene IDs
152831 (Human); 83379 (Mouse); 289625 (Rat); 102147155 (Cynomolgus Monkey)
Alternate Names
betaKlotho; beta-klotho; BKL; KLB; klotho beta like; Klotho beta; klotho beta-like protein; MGC142213

Citation for Recombinant Mouse Klotho beta Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Different roles of N- and C- termini in the functional activity of FGF21.
    Authors: Micanovic R, Raches DW, Dunbar JD, Driver DA, Bina HA, Dickinson CD, Kharitonenkov A
    J. Cell. Physiol., 2009-05-01;219(2):227-34.
    Species: Mouse
    Sample Types: Peptide
    Applications: Surface Plasmon Resonance

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